Recombinant Human TDP1 His Protein

SDS-Page: Recombinant Human TDP1 Protein [NBP2-59661] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.25 mg/ml

Recombinant Human TDP1 His Protein Summary

• Description: A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-608 of Human TDP1
  
  

  Source: Baculovirus

  Amino Acid Sequence: ADPMSQEGDY GRWTISSSDE SEEEKPKPDK PSTSSLLCAR QGAANEPRYT CSEAQKAAHK RKISPVKFSN TDSVLPPKRQ KSGSQEDLGW CLSSSDDELQ PEMPQKQAEK VVIKKEKDIS APNDGTAQRT ENHGAPACHR LKEEEDEYET SGEGQDIWDM LDKGNPFQFY LTRVSGVKPK YNSGALHIKD ILSPLFGTLV SSAQFNYCFD VDWLVKQYPP EFRKKPILLV HGDKREAKAH LHAQAKPYEN ISLCQAKLDI AFGTHHTKMM LLLYEEGLRV VIHTSNLIHA DWHQKTQGIW LSPLYPRIAD GTHKSGESPT HFKADLISYL MAYNAPSLKE WIDVIHKHDL SETNVYLIGS TPGRFQGSQK DNWGHFRLKK LLKDHASSMP NAESWPVVGQ FSSVGSLGAD ESKWLCSEFK ESMLTLGKES KTPGKSSVPL YLIYPSVENV RTSLEGYPAG GSLPYSIQTA EKQNWLHSYF HKWSAETSGR SNAMPHIKTY MRPSPDFSKI AWFLVTSANL SKAAWGALEK NGTQLMIRSY ELGVLFLPSA FGLDSFKVKQ KFFAGSQEPM ATFPVPYDLP PELYGSKDRP WIWNIPYVKA PDTHGNMWVP SHHHHHH

• Source: Baculovirus
• Protein/Peptide Type: Recombinant Protein
• Gene: TDP1
• Purity: >90%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 69.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% glycerol
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human TDP1 His Protein

• EC 3.1.4
• EC 3.1.4.-
• FLJ11090
• MGC104252
• SCAN1
• Tyr-DNA phosphodiesterase 1
• tyrosyl-DNA phosphodiesterase 1

Background

TDP1 is encoded by this gene is involved in repairing stalled topoisomerase I-DNA complexes by catalyzing the hydrolysis of the phosphodiester bond between the tyrosine residue of topoisomerase I and the 3-prime phosphate of DNA. This protein may also remove glycolate from single-stranded DNA containing 3-prime phosphoglycolate, suggesting a role in repair of free-radical mediated DNA double-strand breaks. This gene is a member of the phospholipase D family and contains two PLD phosphodiesterase domains. Mutations in this gene are associated with the disease spinocerebellar ataxia with axonal neuropathy (SCAN1). While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same isoform.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: TDP1