Human TDP1 ELISA Kit (Colorimetric)

ELISA: Human TDP1 ELISA Kit (Colorimetric) [NBP2-60608] - Standard curve.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell Culture
• Standard Curve Range: 1.563 - 100 ng/mL (example only; lot dependent)
• Sensitivity: 1.3 ng/mL (example only; lot dependent)

Human TDP1 ELISA Kit (Colorimetric) Summary

• Description: This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
• Standard Curve Range: 1.563 - 100 ng/mL (example only; lot dependent)
• Sensitivity: 1.3 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 11.6% (example only; lot dependent)
• Intra-Assay: %CV < 5.3% (example only; lot dependent)
• Spike Recovery: 87 - 110% (example only; lot dependent)
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: TDP1

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human TDP1 ELISA Kit (Colorimetric)

• EC 3.1.4
• EC 3.1.4.-
• FLJ11090
• MGC104252
• SCAN1
• Tyr-DNA phosphodiesterase 1
• tyrosyl-DNA phosphodiesterase 1

Background

TDP1 is encoded by this gene is involved in repairing stalled topoisomerase I-DNA complexes by catalyzing the hydrolysis of the phosphodiester bond between the tyrosine residue of topoisomerase I and the 3-prime phosphate of DNA. This protein may also remove glycolate from single-stranded DNA containing 3-prime phosphoglycolate, suggesting a role in repair of free-radical mediated DNA double-strand breaks. This gene is a member of the phospholipase D family and contains two PLD phosphodiesterase domains. Mutations in this gene are associated with the disease spinocerebellar ataxia with axonal neuropathy (SCAN1). While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same isoform.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for TDP1 ELISA Kit (NBP2-60608). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: TDP1