TCR V beta 10b Antibody (B21.5) [PE]

Images

 
Flow Cytometry: TCR V beta 10b Antibody (B21.5) [PE] [NBP2-00266] - Staining of C57Bl/6 splenocytes with Anti-Mouse CD3e APC and 0.125 ug of Rat IgG2a kappa Isotype Control PE (left) or 0.125 ug of Anti-Mouse V beta 10b ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
B21.5
Clonality
Monoclonal
Host
Rat
Concentration
0.2 mg/ml

Order Details

TCR V beta 10b Antibody (B21.5) [PE] Summary

Immunogen
The immunogen for this antibody is TCR V beta 10b.
Isotype
IgG2a Lambda
Clonality
Monoclonal
Host
Rat
Gene
TRB
Purity
Protein A or G purified
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Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Conjugate
PE
Format
PE (A=488, E=575)
Buffer
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
This B21.5 antibody has been tested by flow cytometric analysis of C57BL/6 mouse lymph node cells. This can be used at less than or equal to 0.25 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Reactivity Notes

Mouse

Alternate Names for TCR V beta 10b Antibody (B21.5) [PE]

  • T cell receptor beta locus
  • T-cell antigen receptor, beta polypeptide, T-cell receptor, beta cluster
  • T-cell receptor, beta cluster
  • TCRB
  • TRB

Background

The B21.5 monoclonal antibody reacts with the V-beta10 T-Cell Receptor (TCR) in mice of the beta haplotype of the Tcrb gene complex, which includes AKR, BALB/c, CBA, C3H/He, C57BL, DBA/1 and DBA/2 mice. It does not react with mice having the a or b Tcrb haplotype, including C57BR, C57L or SJL mice.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol TRB

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