TCP1-beta Antibody (PK/8/4/4i/2f)

Images

 
Western Blot: TCP1-beta Antibody (PK/8/4/4i/2f) [NB110-11450] - Analysis of human Jurkat cell lysate probed with TCP-1B mAb (PK/8/4/4i/2f).
Immunohistochemistry: TCP1-beta Antibody (PK/8/4/4i/2f) [NB110-11450] - Human testis tissue stained with TCP-1 B mAb (PK/8/4/4i/2f) at 10ug/ml.
Immunohistochemistry: TCP1-beta Antibody (PK/8/4/4i/2f) [NB110-11450] - Human spleen tissue stained with TCP-1 B, mAb (PK/8/4/4i/2f) at 10ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, Ha, Mk, Rb, ShSpecies Glossary
Applications WB, IHC, IHC-P
Clone
PK/8/4/4i/2f
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.34 mg/ml

Order Details

TCP1-beta Antibody (PK/8/4/4i/2f) Summary

Immunogen
TCP1 beta peptide conjugated to purified tuberculin protein derivative (PPD) (Mouse).
Localization
Cytoplasmic
Specificity
This does not react with human Hsp60 nor with E. coli GroEL proteins.
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
CCT2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
Application Notes
WB: Use at a concentration of 1 ug/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa).
Positive Control
HeLa Lysate (NB800-PC1)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2), 10mM Sodium Phosphate, 50mM NaCl and 0.1mM PMSF
Preservative
No Preservative
Concentration
0.34 mg/ml
Purity
Protein A or G purified

Alternate Names for TCP1-beta Antibody (PK/8/4/4i/2f)

  • Cctb
  • CCT-beta
  • CCTBPRO1633,99D8.1
  • chaperonin containing t-complex polypeptide 1, beta subunit
  • chaperonin containing t-complex polypeptide 1, subunit 2
  • chaperonin containing TCP1, subunit 2 (beta)
  • MGC142074
  • MGC142076
  • T-complex protein 1 subunit beta
  • T-complex protein 1, beta subunit
  • TCP-1-beta

Background

TCP1 beta is a subunit of a cytosolic hetero-oligomer chaperone that is known to be involved in the folding of actin and tubulin. This protein is a member of the chaperonin family, which includes Escherichia coli GroEL, the mitochondrial heat-shock protein Hsp60, the plastid Rubisco-subunit-binding protein and the archaebacterial protein TF55. These chaperonins assist the folding of proteins upon ATP hydrolysis. Nine different subunits of TCP-1 containing chaperonin complexes from mammalian testis and seven different subunits of mouse F9 cells have been identified. It has been suggested that each CCT subunit has a specific, independent function, as they are highly diverged from each other but conserved from mammals to yeast. The expansion in the number of types of CCT subunit, compared with other chaperonins, has allowed CCT to carry out more complex functions that are required for the folding and assembly of highly evolved eukaryotic proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CCT2
Entrez