TCIRG1 Antibody

Immunocytochemistry/Immunofluorescence: TCIRG1 Antibody [NBP1-89333] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: TCIRG1 Antibody [NBP1-89333] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.

Product Discontinued

TCIRG1 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: RPADRQEENKAGLLDLPDASVNGWSSDEEKAGGLDDEEEAELVPSEVLMHQAIHTI
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TCIRG1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22245629).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for TCIRG1 Antibody

• a3
• Atp6i
• ATP6N1C
• ATP6N1Cspecific 116-kDa vacuolar proton pump subunit
• ATP6V0A3T-cell immune response cDNA 7
• OC-116 kDa
• OC116
• OC-116
• OC-116kDa
• OC116Vph1
• OPTB1
• Osteoclastic proton pump 116 kDa subunit
• Stv1
• T-cell immune regulator 1
• T-cell immune response cDNA7 protein
• T-cell, immune regulator 1
• T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein a
• T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A3
• T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein aisoform 3
• T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3
• TCIRG1
• TIRC7
• TIRC7ATPase, H+ transporting, 116kD
• Vacuolar proton translocating ATPase 116 kDa subunit a isoform 3
• vacuolar proton translocating ATPase 116 kDa subunit A
• V-ATPase 116 kDa isoform a3
• V-ATPase 116-kDa
• Vph1
• V-type proton ATPase 116 kDa subunit a isoform 3
• V-type proton ATPase 116 kDa subunit a

Background

TCIRG1 - T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein a isoform 3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TCIRG1 Antibody (NBP1-89333)(4)

Publications using NBP1-89333

• Tejeda-Muñoz N, Azbazdar Y, Monka J et al. The PMA Phorbol Ester Tumor Promoter Increases Canonical Wnt Signaling Via Macropinocytosis bioRxiv : the preprint server for biology 2023-06-05 [PMID: 37333286] (ICC/IF, Human)
• Tejeda-MuNoz N, De Robertis E Lysosomes are Required for Early Dorsal Signaling in the Xenopus Embryo Proc Natl Acad Sci U S A 2022-04-21 [PMID: 35446621]
• Hirata H, Xu X, Nishioka K, et al. PMEPA1 and NEDD4 control the proton production of osteoclasts by regulating vesicular trafficking FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021-02-01 [PMID: 33484199]
• Bronckers AL, Lyaruu DM, Bervoets TJ et al. MURINE AMELOBLASTS ARE IMMUNONEGATIVE FOR TCIRG1, THE V-H-ATPase SUBUNIT ESSENTIAL FOR THE OSTEOCLAST PLASMA MEMBRANE PROTON PUMP. Bone 2012-04-01 [PMID: 22245629] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: TCIRG1