TCFL5 Antibody

Western Blot: TCFL5 Antibody [NB100-53800] - (0.3ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TCFL5 Antibody Summary

• Description: Novus Biologicals Goat TCFL5 Antibody (NB100-53800) is a polyclonal antibody validated for use in WB and ELISA. Anti-TCFL5 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KYIQERHGDSLKKE corresponding to internal region according to NP_006593.2.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TCFL5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 55 kDa band observed in human testis lysates (calculated MW of 52.7 kDa band according to NP_006593.2). An additional band of unknown identity was also consistently observed at 28 kDa. This band was greatly reduced by incubation with the immunizing peptide.
• Control:

  Human Testis Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TCFL5 Antibody

• bHLHe82
• Cha transcription factor
• CHA
• CHAMGC46135
• E2BP1
• E2BP-1HPV-16 E2 binding protein 1
• Figlb
• HPV-16 E2-binding protein 1
• transcription factor-like 5 (basic helix-loop-helix)
• transcription factor-like 5 protein

Background

TCFL5 is a new bHLH transcription factor that negatively regulates upstream transcription factor-dependent transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TCFL5 Antibody (NB100-53800)(1)

Publication using NB100-53800

• Siep M, Sleddens-Linkels E, Mulders S et al. Basic helix-loop-helix transcription factor Tcfl5 interacts with the Calmegin gene promoter in mouse spermatogenesis. Nucleic Acids Res 2004-12-07 [PMID: 15585666]

Bioinformatics

• Gene Symbol: TCFL5
• Entrez:
  • 10732(Human)
  • 277353(Mouse)
• Uniprot:
  • NP_006593.2(Human)