Tau [p Thr231] Antibody (PHF-6)

Product Discontinued

Tau [p Thr231] Antibody (PHF-6) Summary

• Immunogen: Paired helical filaments tau preparation from human brain.
• Modification: p Thr231
• Marker: pan-Neuronal Marker
• Specificity: Reacts with human Tau phosphorylated at threonine 231 and fetal tau. The antibody also reacts with dephosphorylated neurofibrillary tangles. MAB5450 is reactive with the Thr231 phosphorylated and diphosphorylated peptides. No reactivity with normal adult tau or with unphosphorylated or serine 235 phosphorylated protein.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAPT
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:1000
• Application Notes: Western Blot: non-phosphate buffers recommended. Specific for phospho-tau however highly phosphorylated blocking materials like non-fat milk can sometimes cause difficulties, thus we generally recommend blocking western Blots with TBS-1-2% BSA solutions (filtered through a 0.45um membrane) for better results. Immunohistochemistry: fresh frozen tissues with Tris-NaCl-Triton treatment {http://www.jhc.org/cgi/content/full/48/12/1627} & http://ajp.amjpathol.org/cgi/content/full/160/6/2045

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: No Preservative
• Purity: Unpurified

Notes

Relevant Entrez Gene Numbers: NM_005910.3;NM_016834.2;NM_016835.2;NM_016841.2

Alternate Names for Tau [p Thr231] Antibody (PHF-6)

• DDPAC
• FLJ31424
• FTDP-17
• G protein beta1/gamma2 subunit-interacting factor 1
• MAPT
• MGC138549
• microtubule-associated protein tau
• MSTD
• MSTDMAPTL
• MTBT1
• MTBT1Neurofibrillary tangle protein
• MTBT2
• Neurofibrillary tangle protein
• PHF-tau
• PPND
• Tau
• TAUPaired helical filament-tau

Background

Tau is a microtubule binding protein that promotes microtubule assembly and stability. Tau is found to be the major component of the paired helical filaments (PHFs) found in the brains of patients with Alzheimer disease (AD) (1,2). Tau is hyperphosphorylated in PHFs, and specific phosphorylation sites have been implicated in the loss of Tau's association with the membrane cortex during AD disease state, including Ser 199/202, Thr 231, and Ser 393/404 (3). Glycogen synthase kinase-3, or GSK-3, phosphorylates Tau on Ser 396 (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPT
• Uniprot:
  • P10636()