Tau [p Thr231] Antibody (965319) [Unconjugated]

Tauphosphorylated at T231 was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Mouse Anti-Phospho-Tau (T231) Monoclonal Antibody (Catalog # MAB34941) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: All-Multi
• Applications: IHC
• Clone: 965319
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Tau [p Thr231] Antibody (965319) [Unconjugated] Summary

• Immunogen: Phosphopeptide containing the human Tau T231 site
  Accession # P10636
• Modification: p Thr231
• Specificity: Detects human Tau when phosphorylated at T231 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tau [p Thr231] Antibody (965319) [Unconjugated]

• DDPAC
• FLJ31424
• FTDP-17
• G protein beta1/gamma2 subunit-interacting factor 1
• MAPT
• MGC138549
• microtubule-associated protein tau
• MSTD
• MSTDMAPTL
• MTBT1
• MTBT1Neurofibrillary tangle protein
• MTBT2
• Neurofibrillary tangle protein
• PHF-tau
• PPND
• Tau
• TAUPaired helical filament-tau

Background

Tau is a microtubule-associated protein primarily expressed in neurons. It can associate with microtubules through the carboxy-terminal domains and with the plasma membrane through the amino-terminal projection domain. Tau has a role as a stabilizer of microtubules. Abnormal Tau phosphorylation or splicing is associated with various neurological disorders.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Tau Antibody (MAB34941) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human and Mouse.

reviewed by: Marcel Alavi WB Human and Mouse 01/08/2021

Summary

• Application: Western Blot
• Sample Tested: 293T lysate,Mouse Neuro2A whole cell lysate
• Species: Human and Mouse
• Lot: 20200701

Bioinformatics

• Uniprot:
  • P10636()