Human TAT ELISA Kit (Colorimetric)

Product Discontinued

Human TAT ELISA Kit (Colorimetric) Summary

• Description: This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
• Standard Curve Range: 1.481-120 ng/ml
• Sensitivity: 1.1 ng/ml
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 9.9%
• Intra-Assay: CV% < 4.7%
• Spike Recovery: 96%
• Kit Type: ELISA Kit (Colorimetric)
• Gene: TAT

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage is content dependent.

Alternate Names for Human TAT ELISA Kit (Colorimetric)

• EC 2.6.1.5
• L-tyrosine:2-oxoglutarate aminotransferase
• tyrosine aminotransferase
• tyrosine aminotransferase, cytosolic

Background

The transcriptional transactivator (Tat) is a key regulatory protein of HIV. It is expressed early after the virus integrates into the cell, and stimulates the elongation of RNA polymerase II. It binds onto a sequence known as the TAR, or transactivator response element, located at the end of the HIV genetic chain. There, the tat protein helps assemble new copies of HIV. The tat protein-TAR complex speeds up the rate of viral reproduction by about a thousand times. If it is not present, the transcription process frequently stops short, and few functional HIV particles are produced. Tat is an important potential target for antiretrovirals and vaccine development. Transcriptional regulator that acts by binding to the trans-activating responsive sequence (TAR) RNA element and activates transcription initiation and/or elongation from the LTR promoter.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for TAT ELISA Kit (NBP2-60605)(1)

Publication using NBP2-60605

• Krenzlin H, Frenz C, Schmitt J et al. High CSF thrombin concentration and activity is associated with an unfavorable outcome in patients with intracerebral hemorrhage PLoS One 2020-11-11 [PMID: 33175864] (ELISA, Human)
  
  Details:
  ELISA performed on cerebrospinal fluid from human patients who experienced intracerebral hemorrhage.

FAQs for TAT ELISA Kit (NBP2-60605). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: TAT