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TAF3 Antibody

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Western Blot: TAF3 Antibody [NBP2-93431] - Analysis of extracts of various cell lines, using TAF3 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 3min.
Western Blot: TAF3 Antibody [NBP2-93431] - Analysis of extracts of various cell lines, using TAF3 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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TAF3 Antibody Summary

Immunogen
Recombinant protein of human TAF3.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TAF3
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
Theoretical MW
103 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for TAF3 Antibody

  • 140 kDa TATA box-binding protein-associated factor
  • MGC25063
  • RNA polymerase II transcription factor TAFII140
  • TAF(II)140
  • TAF140
  • TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 140kDa
  • TAFII140
  • TAFII-140
  • TBP-associated factor 3
  • transcription initiation factor TFIID 140 kDa subunit

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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TATA binding
protein TBP Antibody
NBP3-13174
Western Blot: TATA binding protein TBP Antibody [NBP3-13174] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TATA binding protein TBP antibody (NBP3-13174) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: TATA binding protein TBP Antibody [NBP3-13174] - TATA binding protein TBP antibody detects TATA binding protein TBP protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. TATA binding protein TBP stained by TATA binding protein TBP antibody (NBP3-13174) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: TATA binding protein TBP Antibody [NBP3-13174] - TATA binding protein TBP antibody detects TATA binding protein TBP protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. TATA binding protein TBP stained by TATA binding protein TBP antibody (NBP3-13174) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
TBPL2 Antibody
NBP1-80268
Western Blot: TBPL2 Antibody [NBP1-80268] - SP2/0 cell lysate, concentration 5.0ug/ml.
Species: Mu
Applications: WB
TAF8 Antibody
NBP2-38188
Immunocytochemistry/Immunofluorescence: TAF8 Antibody [NBP2-38188] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.
Western Blot: TAF8 Antibody [NBP2-38188] - Analysis in control (vector only transfected HEK293T lysate) and TAF8 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: TAF8 Antibody [NBP2-38188] - Staining of human lymph node shows weak nuclear positivity in non-germinal center cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Myogenin Antibody
(5FD) - BSA Free
NB100-56510
Immunohistochemistry-Frozen: Myogenin Antibody (5FD) [NB100-56510] - Analysis of Myogenin in human alveolar rhabdomyosarcoma using NB100-56510 at 5 ug/mL.
Immunohistochemistry: Myogenin Antibody (5FD) [NB100-56510] - Human myocytes using NB100-56703 at 2.5 ug/mL. Hematoxylin-eosin counterstain.
Western Blot: Myogenin Antibody (5FD) [NB100-56510] - Analysis of Myogenin expression in various small-round cell tumor lines. The antibody strongly reacted with a band at ~34 kDa in alveolar rhobdomyosarcoma line (Rh30). Negative cell lines were PFSK-1, EB2, SK-N-SH, and SJSA-1.
(1 Review)
(11 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
IGFBP-rp1/IGFBP-7
Antibody [Unconjugate ...
AF1334
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IGFBP‑rp1/IGFBP‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1334) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IGFBP‑rp1/IGFBP‑7 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
IGFBP‑rp1/IGFBP‑7 was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human IGFBP‑rp1/IGFBP‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1334) at 0.3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Species: Hu
Applications: IHC, WB
TAF10 Antibody
NBP1-80706
Immunocytochemistry/Immunofluorescence: TAF10 Antibody [NBP1-80706] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TAF10 Antibody [NBP1-80706] - Staining of human kidney shows distinct nuclear positivity in glomeruli.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
MyoD Antibody
(5.8A) - BSA Free
NB100-56511
Immunocytochemistry/Immunofluorescence: MyoD Antibody (5.8A) [NB100-56511] - Cell Lines Tested: mouse skeletal muscle-derived primary cell population Test Sample Preparation: mouse skeletal muscle digested by collagenase type 2 System: Super Sensitive High Resolution Confocal Laser Microscope (LSM880 with Airyscan) Excitation Wavelength: 488nm Emission Filter: 562 nm. Image using the DyLight 488 form of this antibody. ICC/IF image submitted by a verified customer review.
Immunocytochemistry/Immunofluorescence: MyoD Antibody (5.8A) [NB100-56511] - Human myoblasts were stained with MyoD1 antibody, diluted 1:200. ICC/IF image submitted by a verified customer review.
Western Blot: MyoD Antibody (5.8A) [NB100-56511] - Insuline-like growth factor (IGF1) antagonized the effects of miR-106a-5p on myogenic differentiation in C2C12. All data were collected from C2C12 myotubes 5 d post differentiation. Western-blot analysis of myogenic regulatory factors (MyoD, MyoG, MyHC) in cells; (H) The statistical results of Figure 3G. Data were presented as mean +/- SEM. n = 3 per group. * p < 0.05, ** p < 0.01. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/2073-4425/9/7/333/htm) licensed under a CC-BY licence. anti-MyoG (catalog# NB100-56510)
(2 Reviews)
(23 Publications)
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO, WB
Histone H3
[Trimethyl Lys9] Anti ...
NBP1-30141
Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Observed molecular weight is ~15 kDa.
Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Representative Western blot showing M-track protein protein proximity signals obtained for Kic1. Hog1-protA-H3: background control, Nup2: positive control. Below: Proximity signals. n = 3 replicates per sample except when indicated differently. Ratios are log2-transformed. Black lines indicate average proximity signal. Proximity signals that differ significantly from background are marked in green (q 0.01) filled circles. Grey filled triangles: q > 0.05. N: N-terminal HKMTmyc fusion. Image collected and cropped by CiteAb from the following publication (https://biosignaling.biomedcentral.com/articles/10.1186/s12964-019-0381-z), licensed under a CC-BY licence.
Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Analysis of Histone H3 K9-me3 in HeLa histone lysates. Observed molecular weight is ~15 kDa.
(2 Reviews)
(24 Publications)
Species: Ce, Dr, Hu, In, Ma, Mu, Po, Rt, Ye
Applications: ChIP, ELISA, ICC/IF, IHC, IHC-P, IP, WB
KAT4/TBP
Associated Factor 1 A
NBP1-77847
Immunohistochemistry: KAT4/TBP Associated Factor 1 Antibody [NBP1-77847] - Tissue: prostate. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-TAF1 at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: TAF-1 as precipitated red signal with hematoxylin purple nuclear counterstain.
Chromatin Immunoprecipitation: KAT4/TBP Associated Factor 1 Antibody [NBP1-77847] - Chromatin Immunoprecipitation: KAT4/TBP Associated Factor 1 Antibody [NBP1-77847] Transcription Initiation Factor TFIID Subunit 1 (TAF1) antibody was used to detect TAF1 in treated and untreated HeLa Cells. HeLa cells were treated with TNF alpha and Chromatin was prepared by Chip Kit. CHIP was performed on fos promoters using 5 ug of TAF1 antibody and an RNA PolII antibody.
Western Blot: KAT4/TBP Associated Factor 1 Antibody [NBP1-77847] - HeLa nuclear extract (arrowhead). The membrane was probed with the primary antibody at dilutions of 1:100 (lane 1), 1:250 (lane 2), 1: 500 (Lane 3 and 1:1,000 (Lane 4). The identity of the bands at ~95 kDa is unknown, but may be degraded TAF1. Personal Communication, Anne Gegonne, CCR-NCI, Bethesda, MD.
Species: Hu, Mu
Applications: ChIP, ELISA, IHC, IHC-P, IP, WB
Taf4 Antibody
NBP1-80705
Immunocytochemistry/Immunofluorescence: Taf4 Antibody [NBP1-80705] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: Taf4 Antibody [NBP1-80705] - Staining of human duodenum shows moderate nuclear and cytoplasmic positivity in glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
TAF7 Antibody (2C5)
H00006879-M01
Immunocytochemistry/Immunofluorescence: TAF7 Antibody (2C5) [H00006879-M01] - Analysis of monoclonal antibody to TAF7 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: TAF7 Antibody (2C5) [H00006879-M01] - Analysis of monoclonal antibody to TAF7 on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 3 ug/ml.
Immunohistochemistry-Paraffin: TAF7 Antibody (2C5) [H00006879-M01] - Analysis of monoclonal antibody to TAF7 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 1 ug/ml.
(5 Publications)
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-P, KD, WB
TAF1D Antibody
NBP2-31616
Immunohistochemistry-Paraffin: TAF1D Antibody [NBP2-31616] - Staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.
Immunocytochemistry/Immunofluorescence: TAF1D Antibody [NBP2-31616] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm. Antibody staining is shown in green.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
HMGA1 Antibody
(5J0E6)
NBP3-16379
Immunohistochemistry-Paraffin: HMGA1 Antibody (5J0E6) [NBP3-16379] - Immunohistochemistry of paraffin-embedded rat brain using HMGA1 Rabbit mAb (NBP3-16379) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: HMGA1 Antibody (5J0E6) [NBP3-16379] - Immunohistochemistry of paraffin-embedded human oophoroma using HMGA1 Rabbit mAb (NBP3-16379) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunocytochemistry/Immunofluorescence: HMGA1 Antibody (5J0E6) [NBP3-16379] - Immunofluorescence analysis of C6 cells using HMGA1 Rabbit mAb (NBP3-16379) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Species: Hu, Mu, Rt
Applications: ChIP, ICC/IF, IHC, IHC-P, IP, WB
CHAF1A Antibody
(SS 1 1-13)
NB500-207
Western Blot: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Analysis of CAF1 p150 expression in Ntera2 whole cell lysate using NB500-207.
Immunocytochemistry/Immunofluorescence: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Immunofluorescent staining of CAF-1-p150 in HeLa cells (Shibahara et al 1999).
(10 Publications)
Species: Hu
Applications: ICC/IF, IP, WB
TAF15 Antibody
(4D71)
NBP1-92685
Immunocytochemistry/Immunofluorescence: TAF15 Antibody (4D71) [NBP1-92685] - Analysis of HeLa cells stained with mouse mAb to TATA-box binding protein associated factor 15, TAF15, NBP1-92685, dilution 1:500 in red, and costained with rabbit pAb to GAPDH, dilution 1:1,000 in green. Blue is Hoechst staining of nuclear DNA. The NBP1-92685 antibody reveals TAF15 protein expressed exclusively in the nuclei of the cells, while GAPDH antibody produces strong cytoplasmic staining.
Western Blot: TAF15 Antibody (4D71) [NBP1-92685] - Analysis of cytosol or nuclear enriched fractions of cell line lysates using mouse mAb to TAF15, NBP1-92685, dilution 1:1,000: [1] protein standard, [2] NIH-3T3 cytosol, [3] NIH-3T3 nuclear fraction, [4] HeLa cytosol, and [5] HeLa nuclear fraction.Double band at 56-68kDa detected predominantly in the nuclear enriched fraction corresponds to the TAF15 protein.
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, WB
CNOT7 Antibody
(2F6)
H00029883-M01
Immunocytochemistry/Immunofluorescence: CNOT7 Antibody (2F6) [H00029883-M01] - Analysis of monoclonal antibody to CNOT7 on HeLa cell. Antibody concentration 40 ug/ml.
Sandwich ELISA: CNOT7 Antibody (2F6) [H00029883-M01] - Detection limit for recombinant GST tagged CNOT7 is 3 ng/ml as a capture antibody.
Western Blot: CNOT7 Antibody (2F6) [H00029883-M01] - CNOT7 monoclonal antibody (M01), clone 2F6 Analysis of CNOT7 expression in HeLa.
(5 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, S-ELISA, WB
SUPT7L Antibody
NBP2-57671
Western Blot: SUPT7L Antibody [NBP2-57671] - Western blot analysis in human cell line RT-4, human cell line U-251 MG and human plasma.
Immunocytochemistry/Immunofluorescence: SUPT7L Antibody [NBP2-57671] - Staining of human cell line SH-SY5Y shows localization to nucleoplasm.
Species: Hu
Applications: ICC/IF, WB
CD1e Antibody
(704407) [Unconjugate
MAB7330
Jurkat human acute T cell leukemia cell line was treated for 3 hours with monensin, then stained with Mouse Anti-Human CD1e Monoclonal Antibody (Catalog # MAB7330, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).  To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. 
Species: Hu
Applications: CyTOF-ready, ICFlow
TAF148 Antibody
NBP2-47609
Immunocytochemistry/Immunofluorescence: TAF148 Antibody [NBP2-47609] - Staining of human cell line A-431 shows localization to nucleoplasm.
Immunohistochemistry: TAF148 Antibody [NBP2-47609] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
TAF3 Antibody
NBP2-93431
TAF3 Antibody
Species: Hu, Mu, Rt
Applications: WB

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Bioinformatics

Gene Symbol TAF3