TACC3 Antibody (SN73-05)

Western Blot: TACC3 Antibody (SN73-05) [NBP2-67671] - Western blot analysis of TACC3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab

Immunocytochemistry/Immunofluorescence: TACC3 Antibody (SN73-05) [NBP2-67671] - Staining TACC3 in PMVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: TACC3 Antibody (SN73-05) [NBP2-67671] - Analysis of paraffin-embedded mouse testis tissue using anti-TACC3 antibody. Counter stained with hematoxylin.

Western Blot: TACC3 Antibody (SN73-05) [NBP2-67671] - Analysis of TACC3 on Hela cells lysates using anti-TACC3 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: TACC3 Antibody (SN73-05) [NBP2-67671] - Staining TACC3 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: TACC3 Antibody (SN73-05) [NBP2-67671] - Staining TACC3 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: TACC3 Antibody (SN73-05) [NBP2-67671] - Analysis of paraffin-embedded human tonsil tissue using anti-TACC3 antibody. Counter stained with hematoxylin.

Product Discontinued

TACC3 Antibody (SN73-05) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human TACC3 aa 35-84 / 838. (SwissProt: Q9Y6A5 Human; SwissProt: Q9JJ11 Mouse; Unigene:58838 Rat)
• Localization: Centrosome, spindle, spindle pole, Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TACC3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TACC3 Antibody (SN73-05)

• ERIC-1
• ERIC1ERIC-1
• MGC117382
• MGC133242
• TACC3
• transforming acidic coiled-coil-containing protein 3
• transforming, acidic coiled-coil containing protein 3

Background

The function of this gene has not yet been determined; however, it is speculated that it may be involved in cell growth and differentiation. Expression of this gene is up-regulated in some cancer cell lines, and in embryonic day 15 in mice.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TACC3 Antibody (NBP2-67671)(1)

Publication using NBP2-67671

• Blengini C, Ibrahimian P, Vaskovicova M, et al. Aurora kinase A is essential for meiosis in mouse oocytes PLoS Genet 2021-04-26 [PMID: 33901174]

Bioinformatics

• Gene Symbol: TACC3