Synuclein alpha/beta Antibody (ST05-21)

Western Blot: Synuclein alpha/beta Antibody (ST05-21) [NBP2-67537] - Western blot analysis of Synuclein alpha/beta on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for

Immunocytochemistry/Immunofluorescence: Synuclein alpha/beta Antibody (ST05-21) [NBP2-67537] - Staining alpha+beta Synuclein in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Synuclein alpha/beta Antibody (ST05-21) [NBP2-67537] - Analysis of paraffin-embedded rat brain tissue using anti-alpha+beta Synuclein antibody. Counter stained with hematoxylin.

Flow Cytometry: Synuclein alpha/beta Antibody (ST05-21) [NBP2-67537] - Flow cytometric analysis of Synuclein alpha/beta was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).

Immunocytochemistry/Immunofluorescence: Synuclein alpha/beta Antibody (ST05-21) [NBP2-67537] - Staining alpha+beta Synuclein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Synuclein alpha/beta Antibody (ST05-21) [NBP2-67537] - Staining alpha+beta Synuclein in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Synuclein alpha/beta Antibody (ST05-21) [NBP2-67537] - Analysis of paraffin-embedded mouse brain tissue using anti-alpha+beta Synuclein antibody. Counter stained with hematoxylin.

Flow Cytometry: Synuclein alpha/beta Antibody (ST05-21) [NBP2-67537] - Analysis of Hela cells with alpha+beta Synuclein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibod

Product Discontinued

Synuclein alpha/beta Antibody (ST05-21) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Synuclein alpha/beta aa 2-50 / 140. (SwissProt: P37840 Human; SwissProt: Q16143 Human; SwissProt: O55042 Mouse; SwissProt: Q91ZZ3 Mouse; SwissProt: P37377 Rat; SwissProt: Q63754 Rat)
• Localization: Cytoplasm, Nucleus, Membrane, Secreted, Cell junction.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SNCA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Synuclein alpha/beta Antibody (ST05-21)

• beta-synuclein
• I(2)-synuclein
• I+/--synuclein
• NACP
• non A-beta component of AD amyloid
• PARK1
• PD1
• synuclein alpha-140
• synuclein, alpha (non A4 component of amyloid precursor)
• truncated alpha synuclein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SNCA