Syntenin 1 Antibody (OTI2H6) - Azide and BSA Free

Western Blot: Syntenin 1 Antibody (OTI2H6) - Azide and BSA Free [NBP2-74418] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Syntenin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Syntenin 1.

Immunohistochemistry: Syntenin 1 Antibody (OTI2H6) - Azide and BSA Free [NBP2-74418] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Syntenin 1 mouse monoclonal antibody.

Western Blot: Syntenin 1 Antibody (OTI2H6) - Azide and BSA Free [NBP2-74418] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-Syntenin 1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry: Syntenin 1 Antibody (OTI2H6) - Azide and BSA Free [NBP2-74418] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Syntenin 1 mouse monoclonal antibody.

Immunohistochemistry: Syntenin 1 Antibody (OTI2H6) - Azide and BSA Free [NBP2-74418] - Staining of paraffin-embedded Human liver tissue using anti-Syntenin 1 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI2H6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Syntenin 1 Antibody (OTI2H6) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Syntenin 1 Antibody (OTI2H6) - Azide and BSA Free (NBP2-02072) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human SDCBP(NP_005616) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SDCBP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
• Theoretical MW: 32.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Syntenin 1 Antibody (OTI2H6) - Azide and BSA Free

• MDA9
• MDA-9
• melanoma differentiation associated protein-9
• Melanoma differentiation-associated protein 9
• Pro-TGF-alpha cytoplasmic domain-interacting protein 18
• Scaffold protein Pbp1
• ST1
• SYCLTACIP18
• syndecan binding protein (syntenin)
• Syndecan-binding protein 1
• syntenin-1

Background

Syntenin 1 is encoded by this gene was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. The syntenin protein contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. This protein may also affect cytoskeletal-membrane organization, cell adhesion, protein trafficking, and the activation of transcription factors. The protein is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SDCBP