Syntenin 1 Antibody (GT1523) - Azide and BSA Free

Western Blot: Syntenin 1 Antibody (GT1523) [NBP3-13586] - HepG2 whole cell and exosome extracts (1.8 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody [GT1523] (NBP3-13586) diluted at 1:250. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Syntenin 1 Antibody (GT1523) [NBP3-13586] - Syntenin 1 antibody [GT1523] detects Syntenin 1 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: Syntenin 1 protein stained by Syntenin 1 antibody [GT1523] (NBP3-13586) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.

Western Blot: Syntenin 1 Antibody (GT1523) [NBP3-13586] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody [GT1523] (NBP3-13586) diluted at 1:5000.

Western Blot: Syntenin 1 Antibody (GT1523) [NBP3-13586] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody [GT1523] (NBP3-13586) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

• Reactivity: Hu, Rt, Mu, Po, Bv, Ch, Pm, RM, Xp
• Applications: WB, ICC/IF
• Clone: GT1523
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Syntenin 1 Antibody (GT1523) - Azide and BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Syntenin 1. The exact sequence is proprietary.
• Predicted Species: Mouse (100%), Porcine (100%), Chimpanzee (100%), Bovine (100%), Rhesus Macaque (100%), Chicken (100%), Xenopus (100%). Backed by our 100% Guarantee.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SDCBP
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:10000
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Syntenin 1 Antibody (GT1523) - Azide and BSA Free

• MDA9
• MDA-9
• melanoma differentiation associated protein-9
• Melanoma differentiation-associated protein 9
• Pro-TGF-alpha cytoplasmic domain-interacting protein 18
• Scaffold protein Pbp1
• ST1
• SYCLTACIP18
• syndecan binding protein (syntenin)
• Syndecan-binding protein 1
• syntenin-1

Background

Syntenin 1 is encoded by this gene was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. The syntenin protein contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. This protein may also affect cytoskeletal-membrane organization, cell adhesion, protein trafficking, and the activation of transcription factors. The protein is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SDCBP