Syntaxin 6 Antibody (3D10)

Western Blot: Syntaxin-6 Antibody (3D10) [NBP1-54478] - Western blot analysis of syntaxin-6 expression in mouse brain (1), rat brain (2), WR19L cells (3) and PC12 cells (4) using NBP1-54478.

Immunoprecipitation: Syntaxin 6 Antibody (3D10) [NBP1-54478] - Mouse brain with (NBP1-54478) (1) or normal mouse IgG (2). After immunoprecipitated with the antibody, immunocomlex was resolved on SDS-Page and immunoblotted with (NBP1-54478).

Product Discontinued

Syntaxin 6 Antibody (3D10) Summary

• Immunogen: A hybridoma was established by fusion of mouse myeloma cell NS-1 with Balb/c mouse splenocyte immunized with the recombinant rat syntaxin-6.
• Marker: TGN Marker
• Specificity: It is reported that this monoclonal antibody (3D10) binds to the amino-terminal 25 amino acid of rat syntaxin-6.
• Predicted Species: Human (95%). Backed by our 100% Guarantee.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: STX6
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 ug/300 uL of cell extract
  • Western Blot 1 ug/ml

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 50% Glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Syntaxin 6 Antibody (3D10)

• STX6
• Syntaxin 6
• syntaxin-6

Background

Syntaxin 6 is a 35 kDa type II integral membrane protein with two coiled coil domains and a carboxyl terminal membrane anchor. Immunoelectron microscopy studies show that syntaxin 6 is primarily localized to the trans Golgi network (TGN), and is partially co localized with AP 1 on clathrin coated membranes. During secretory granule maturation, syntaxin 6 and MPR ligand complexes are postulated to be transferred from immature granules to endosomes by clathrin coated vesicles, as evidenced by immunoelectron microscopy. These results suggest that syntaxin 6 mediates a TGN trafficking targeting endosome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STX6
• Entrez:
  • 60562(Rat)
• Uniprot:
  • Q63635(Rat)