Syntaxin 6 Antibody (3D10)

Images

 
Western Blot: Syntaxin-6 Antibody (3D10) [NBP1-54478] - Western blot analysis of syntaxin-6 expression in mouse brain (1), rat brain (2), WR19L cells (3) and PC12 cells (4) using NBP1-54478.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IP, IHC (-)
Clone
3D10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Syntaxin 6 Antibody (3D10) Summary

Immunogen
A hybridoma was established by fusion of mouse myeloma cell NS-1 with Balb/c mouse splenocyte immunized with the recombinant rat syntaxin-6.
Marker
TGN Marker
Specificity
It is reported that this monoclonal antibody (3D10) binds to the amino-terminal 25 amino acid of rat syntaxin-6.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
STX6
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 2 ug/300 uL of cell extract

Reactivity Notes

Hamster, Mouse, Rat. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Syntaxin 6 Antibody (3D10)

  • STX6
  • Syntaxin 6
  • syntaxin-6

Background

Syntaxin 6 is a 35 kDa type II integral membrane protein with two coiled coil domains and a carboxyl terminal membrane anchor. Immunoelectron microscopy studies show that syntaxin 6 is primarily localized to the trans Golgi network (TGN), and is partially co localized with AP 1 on clathrin coated membranes. During secretory granule maturation, syntaxin 6 and MPR ligand complexes are postulated to be transferred from immature granules to endosomes by clathrin coated vesicles, as evidenced by immunoelectron microscopy. These results suggest that syntaxin 6 mediates a TGN trafficking targeting endosome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol STX6
Entrez
Uniprot