Syntaxin 1A Antibody

Images

 
Western Blot: Syntaxin 1A Antibody [NB600-818] - Analysis of Syntaxin 1A in Mouse Brain lysate using NB600-818 at 0.003 ug/ml.
Western Blot: Syntaxin 1A Antibody [NB600-818] - Staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Syntaxin 1A Antibody Summary

Immunogen
Peptide with sequence KDRTQELRTAKD-C corresponding to N-Terminus according to NP_001159375.1, NP_443106.1.
Specificity
Please note this product is expected to recognize the products of 2 different genes. STX1A (NP_004594.1and NP_001159375.1) and STX1B (NP_443106.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
STX1A
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.01 - 0.03 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 35 kDa band observed in mouse brain lysates (calculated MW of 33.0 kDa band according to NP_004594.1 and 33.2 kDa band according to NP_443106.1).
Positive Control
Brain Lysate (NB820-59657)
Publications
Read Publication using NB600-818.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Human, Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Syntaxin 1A Antibody

  • Brain Syntaxin
  • HPC-1
  • Neuron-specific antigen HPC-1
  • p35-1
  • STX1A
  • STX1P35-1
  • SYN1A
  • syntaxin 1A (brain)
  • Syntaxin 1A
  • syntaxin-1A

Background

Please note this product is expected to recognize the products of 2 different genes. Synaptic vesicles store neurotransmitters that are released during calcium-regulated exocytosis. The specificity of neurotransmitter release requires the localization of both synaptic vesicles and calcium channels to the presynaptic active zone. Syntaxins function in this vesicle fusion process. Syntaxins also serve as a substrate for botulinum neurotoxin type C, a metalloprotease that blocks exocytosis and has high affinity for a molecular complex that includes the alpha-latrotoxin receptor (MIM 600565) which produces explosive exocytosis (Zhang et al., 1995).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Syntaxin 1A Antibody (NB600-818)(1)

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol STX1A
Entrez
Uniprot