Syntaxin 12 Antibody (677201)

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Western blot shows lysates of CCD‑1070Sk human foreskin fibroblast cell line, LNCaP human prostate cancer cell line, and MDA‑MB‑453 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Mouse ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
677201
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Syntaxin 12 Antibody (677201) Summary

Immunogen
E. coli-derived recombinant human Syntaxin 12
Ser2-Gln200
Accession # Q86Y82
Specificity
Detects human Syntaxin 12 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Syntaxin 1A, 1B1, 1B2, 5, 6, 7, 8, or 16 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
STX12
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Syntaxin 12 Antibody (677201)

  • MGC51957
  • STX12
  • STX13
  • STX14
  • Syntaxin 12
  • syntaxin-12

Background

Syntaxin 12/STX12 is a 39 kDa member of the Syntaxin family. It is a SNARE that acts to regulate protein transport between late endosomes and the trans-Golgi network. Human STX12 is 276 amino acids (aa) in length, and is a type IV single-pass transmembrane protein. The cytoplasmic region extends from aa 1-248. A coiled-coil region extends from aa 33-131 within that region. From aa 178-240, there is a t-SNARE coiled-coil homology domain. The transmembrane region is located at 249-269, and a vesicular area is located at aa 270-276. Human STX12 shares 92% aa sequence identity with mouse STX12.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol STX12
Uniprot