SUV420h1 Antibody [DyLight 650]

Flow Cytometry: SUV420h1 Antibody [DyLight 650] [NBP1-97313C] - An intracellular stain was performed on SK-MEL-28 cells with SUV420h1 Antibody NBP1-97313C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.

• Reactivity: Hu, Mu
• Applications: Flow, ICC/IF, IHC, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: DyLight 650

SUV420h1 Antibody [DyLight 650] Summary

• Immunogen: A synthetic peptide made to an internal portion of the human SUV420h1 protein (between residues 450-500) [UniProt Q4FZB7]
• Localization: Nucleus. Chromosome. Note: Associated with pericentric heterochromatin. CBX1 and CBX5 are required for the localization to pericentric heterochromatin.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KMT5B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 5 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for SUV420h1 Antibody [DyLight 650]

• C630029K18Rik
• CGI85
• CGI-85
• EC 2.1.1.43
• histone-lysine N-methyltransferase SUV420H1
• KMT5B Lysine N-methyltransferase 5B
• KMT5B
• MGC118906
• MGC118909
• MGC21161
• MGC703
• Su(var)4-20 homolog 1
• suppressor of variegation 4-20 homolog 1 (Drosophila)
• Suppressor of variegation 4-20 homolog 1
• SUV420h1
• Suv4-20h1

Background

SUV420H1 (Histone-lysine N-methyltransferase 5B, KMT5B) is a histone methyltransferase that specifically di- and trimethlyates lysine 20 of H4 (H4 K20Me3), an epigenetic mark of transcriptional repression. This mark, along with trimethylation of histone 3 lysine 9 (H3 K9me3) and monomethylation of H3 lysine 27 (H3 K27me1), define genomic regions of constitutive heterochromatin that are poor in activated genes. This enzyme is essential for development, with Suv420h1 knockout mice proving lethal perinatally and showing overall growth retardation. The RB1 family of proteins have been shown to interact with, and target, KMT5B to histones, enabling the enzyme to function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KMT5B