SUPT16H Antibody (1D12) - Azide and BSA Free

Western Blot: SUPT16H Antibody (1D12) [H00011198-M01] - SUPT16H monoclonal antibody (M01), clone 1D12 Analysis of SUPT16H expression in Hela S3 NE.

Sandwich ELISA: SUPT16H Antibody (1D12) [H00011198-M01] - Detection limit for recombinant GST tagged SUPT16H is 0.03 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 1D12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SUPT16H Antibody (1D12) - Azide and BSA Free Summary

• Immunogen: SUPT16H (NP_009123, 608 a.a. ~ 715 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PGEQTVPALNLQNAFRIIKEVQKRYKTREAEEKEKEGIVKQDSLVINLNRSNPKLKDLYIRPNIAQKRMQGSLEAHVNGFRFTSVRGDKVDILYNNIKHALFQPCDGE
• Specificity: suppressor of Ty 16 homolog (S. cerevisiae)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SUPT16H
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SUPT16H Antibody (1D12) - Azide and BSA Free

• CDC68
• Chromatin-specific transcription elongation factor 140 kDa subunit
• facilitates chromatin remodeling 140 kDa subunit
• Facilitates chromatin transcription complex subunit SPT16
• FACT 140 kDa subunit
• FACT complex subunit SPT16
• FACT140
• FACTp140
• FACTP140FACT
• FLJ10857
• FLJ14010
• FLJ34357
• hSPT16
• SPT16/CDC68
• suppressor of Ty (S.cerevisiae) 16 homolog
• suppressor of Ty 16 homolog (S. cerevisiae)

Background

Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit, the latter of which is the protein encoded by this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SUPT16H Antibody (H00011198-M01)(1)

Publication using H00011198-M01

• AV Luzhin, B Avanesyan, AK Velichko, VO Shender, N Ovsyanniko, GP Arapidi, PV Shnaider, NV Petrova, II Kireev, SV Razin, OL Kantidze Chromatin Trapping of Factors Involved in DNA Replication and Repair Underlies Heat-Induced Radio- and Chemosensitization Cells, 2020-06-08;9(6):. 2020-06-08 [PMID: 32521766]

Bioinformatics

• Gene Symbol: SUPT16H
• Entrez:
  • 11198(Human)
• OMIM:
  • 605012(Human)
• Uniprot:
  • NP_009123(Human)