SUMO Activating Enzyme E1 (SAE1) Antibody (JG35-88)

Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody (JG35-88) [NBP2-76866] - Western blot analysis of SAE1 on different cell lysates using anti-SAE1 antibody at 1/1,000 dilution.Positive control:Lane 1: SiHa Lane 2: K562Lane 3: 293

Flow Cytometry: SUMO Activating Enzyme E1 (SAE1) Antibody (JG35-88) [NBP2-76866] - Flow cytometric analysis of A549 cells with SAE1 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Product Discontinued

SUMO Activating Enzyme E1 (SAE1) Antibody (JG35-88) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human SUMO Activating Enzyme E1 (SAE1) aa 201-249 / 346. (SwissProt: Q9UBE0 Human)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SAE1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Western Blot 1:500-1:2000
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SUMO Activating Enzyme E1 (SAE1) Antibody (JG35-88)

• AOS1
• AOS1activator of SUMO1
• FLJ3091
• HSPC140
• SAE1
• SUA1
• SUA1sentrin/SUMO-activating protein AOS1
• SUMO Activating Enzyme E1 (SAE1/UBA2)
• SUMO-1 activating enzyme E1 N subunit
• SUMO1 activating enzyme subunit 1
• SUMO-1 activating enzyme subunit 1
• SUMO-activating enzyme subunit 1
• UBA2
• Ubiquitin-like 1-activating enzyme E1A
• ubiquitin-like protein SUMO-1 activating enzyme
• UBLE1A

Background

The dimeric enzyme acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SAE1