Recombinant Human SUGT1 Protein

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SDS-Page: Recombinant Human SUGT1 Protein [NBP1-50979] - 15 % SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human SUGT1 Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 115-365 of Human SUGT1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MHRVGQAGLQ LLTSSDPPAL DSQSAGITGA DANFSVWIKR CQEAQNGSES EVWTHQSKIK YDWYQTESQV VITLMIKNVQ KNDVNVEFSE KELSALVKLP SGEDYNLKLE LLHPIIPEQS TFKVLSTKIE IKLKKPEAVR WEKLEGQGDV PTPKQFVADV KNLYPSSSPY TRNWDKLVGE IKEEEKNEKL EGDAALNRLF QQIYSDGSDE VKRAMNKSFM ESGGTVLSTN WSDVGKRKVE INPPDDMEWK KY

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
SUGT1
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
30.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT, 0.1M NaCl
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human SUGT1 Protein

  • Protein 40-6-3
  • putative 40-6-3 protein
  • SGT1
  • SGT1, suppressor of G2 allele of SKP1 (S. cerevisiae)
  • SGT1B protein
  • suppressor of G2 allele of SKP1 homolog
  • suppressor of G2 allele of SKP1, S. cerevisiae, homolog of

Background

SUGT1, also known as suppressor of G2 allele of SKP1, is a homolog of the yeast protein SGT1, a regulator of the cell cycle that is essential for G1/S and G2/M transitions. It contains a CS domain, a SGS domain, a p23 domain and three tetratricopeptide repeats (TPR). This protein associates with Skp1 p19 and CUL-1, subunits of the SCF ubiquitin ligase complex, and is thought to play a role in protein degradation. In addition, it is required for the kinetochores assembly, and has function as a co-chaperone for HSP90. Recombinant human SUGT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol SUGT1