Recombinant Human SUGT1 His Protein

SDS-Page: Recombinant Human SUGT1 Protein [NBP1-50979] - 15 % SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human SUGT1 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 115-365 of Human SUGT1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MHRVGQAGLQ LLTSSDPPAL DSQSAGITGA DANFSVWIKR CQEAQNGSES EVWTHQSKIK YDWYQTESQV VITLMIKNVQ KNDVNVEFSE KELSALVKLP SGEDYNLKLE LLHPIIPEQS TFKVLSTKIE IKLKKPEAVR WEKLEGQGDV PTPKQFVADV KNLYPSSSPY TRNWDKLVGE IKEEEKNEKL EGDAALNRLF QQIYSDGSDE VKRAMNKSFM ESGGTVLSTN WSDVGKRKVE INPPDDMEWK KY

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: SUGT1
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 30.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 1 mM DTT, 0.1 M NaCl
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human SUGT1 His Protein

• Protein 40-6-3
• putative 40-6-3 protein
• SGT1
• SGT1, suppressor of G2 allele of SKP1 (S. cerevisiae)
• SGT1B protein
• suppressor of G2 allele of SKP1 homolog
• suppressor of G2 allele of SKP1, S. cerevisiae, homolog of

Background

SUGT1, also known as suppressor of G2 allele of SKP1, is a homolog of the yeast protein SGT1, a regulator of the cell cycle that is essential for G1/S and G2/M transitions. It contains a CS domain, a SGS domain, a p23 domain and three tetratricopeptide repeats (TPR). This protein associates with Skp1 p19 and CUL-1, subunits of the SCF ubiquitin ligase complex, and is thought to play a role in protein degradation. In addition, it is required for the kinetochores assembly, and has function as a co-chaperone for HSP90. Recombinant human SUGT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: SUGT1