Substance P Antibody (NC1/34) - BSA Free

Immunohistochemistry-Frozen: Substance P Antibody (NC1/34) [NB100-65219] - Opioid R/OPRM1 (green) and Substance P (red) were detected in frozen mouse spinal cord and DRG tissue by fluorescent multiplex IHC. Mu Opioid R/OPRM1 Antibody (NB100-1620), used at 1:100, and Substance P Antibody (NB100-65219), used at 1:50, were applied to the tissue and simultaneously incubated at 4C overnight. Anti-Rabbit Alexa Fluor(R) 488-conjugated secondary antibody and anti-Rat NL557-conjugated secondary antibody (NL013) were applied to the tissue, and simultaneously incubated at room temperature, protected from light for 1 hour. Coverslips were applied to the tissue slide along with mounting media containing DAPI (blue) and imaged using a 4X objective lens.

• Reactivity: All-Multi
• Applications: IHC
• Clone: NC1/34
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free

Substance P Antibody (NC1/34) - BSA Free Summary

• Description: Novus Biologicals Rat Substance P Antibody (NC1/34) - BSA Free (NB100-65219) is a monoclonal antibody validated for use in IHC. Anti-Substance P Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Substance P conjugated to bovine serum albumin
• Specificity: NB100-65219 recognizes the COOH terminal end of substance P, a short polypeptide neurotransmitter that regulates the excitability of dorsal horn nociceptive neurons. It is known to have a role in several physiologic activities such as pain transmission, the vomiting reflex, salivary secretion and smooth muscle contraction. 5% reactivity is observed with eledoisin. It does not react with Leu or Met-enkephalin, somatostatin or beta-endorphin.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: TAC1
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:100 - 1:200
  • Immunohistochemistry-Paraffin 1:100 - 1:200
• Application Notes: This product does not require antigen retrieval prior to staining of IHC-P sections. This antibody has been used for the localization of substance P in tissue sections by immunofluorescence and PAP staining in nervous systems of experimental animals and man. Please refer to the Cuello references listed below. It has also been successfully applied in archive post-mortem tissues of the human brain, see Mai and Pioro references. Use in Immunohistochemistry whole mount reported in scientific literature (PMID 24728885)

Reactivity Notes

Predicted cross-reactivities: Guinea Pig, Homarus sp., Human, Mammals, Lamprey, Bichir, Beaver, Amphibia, Bovine, Camel, Cancer sp., Calanus sp., Bullfrog, Cowbird, Crustacea, Crayfish, Chicken, Cockroach, Chinchilla, Chinchilla, Porcine, Porcineeon, Rabbit, Mudpuppy, Panilurus sp., Sheep, Rat, Spadefoot toad, Zebrafish

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tissue culture supernatant
• Preservative: 0.09% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for Substance P Antibody (NC1/34) - BSA Free

• neurokinin 1
• neurokinin 2
• neurokinin A
• Neurokinin-1
• neuromedin L, neurokinin alpha, neuropeptide K, neuropeptide gamma)
• neuropeptide gamma
• neuropeptide K
• NK2
• NKA
• NKNAneurokinin alpha
• NPK
• PPT
• protachykinin-1
• substance K
• Substance P
• TAC1
• TAC2neuromedin L
• tachykinin 2
• tachykinin, precursor 1 (substance K, substance P, neurokinin 1, neurokinin 2
• tachykinin, precursor 1

Background

Substance P (SP) is currently thought to be one of the most important neurotransmitters and neurotransmitters in the brain. It is an undecapeptide belonging to the tachykinin family, which consists of excitatory neuropeptides synthesized in neuronal and glial cells of the human central and peripheral nervous system. Substance P, Neurokinin A, Neuropeptide K, and Neuropeptide gamma are all generated by post-translation cleavage of the precursor Protachykinin-1. Substance P forms the major endogenous ligand for Neurokinin 1 Receptor. The pharmacology of Substance P has been associated with a number of neurological and psychiatric disorders, namely nociception, migraine, asthma, nausea, inflammatory bowel syndrome, urinary incontinence, anxiety and depression. It has also been linked to obesity. This antibody reacts with substance P, a bioactive peptide secreted from neurons both in the gut and central nervous system. Can be used for the localisation of Substance P in cells and tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Substance P Antibody (NB100-65219)(3)

Publications using NB100-65219

• Gutjahr R, Bothe M, Chagnaud B Immunohistochemical Profile of a Conspicuously Organized Structure in the Dorsal Forebrain of the Peacock Gudgeon, Tateurndina ocellicauda. The Journal of comparative neurology 2025-10-16 [PMID: 41094659]
• Jagadeeshaprasad MG, Govindappa PK, Nelson AM et al. 4-Aminopyridine Induces Nerve Growth Factor to Improve Skin Wound Healing and Tissue Regeneration Biomedicines 2022-07-08 [PMID: 35884953] (Immunohistochemistry-Frozen, Immunohistochemistry Whole-Mount, Mouse)
• Pelayo JC, Veldhuis NA, Eriksson EM et al. Localisation and activation of the neurokinin 1 receptor in the enteric nervous system of the mouse distal colon. Cell Tissue Res. 2014-04-12 [PMID: 24728885] (IHC-Fr, IHC-WhMt, Mouse)

Review for Substance P Antibody (NB100-65219) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Giulia Di Cristina IHC-Fr Other 07/21/2016

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Arm section
• Species: Other
• Lot: 080514

Bioinformatics

• Gene Symbol: TAC1
• Entrez:
  • 6863(Human)
• Uniprot:
  • P20366(Human)