STK35 Antibody

Images

 
Western Blot: STK35 Antibody [NB100-1393] - Staining of nuclear HEK293 with antibody at 2 ug/mL (A) and Human Breast cancer with antibody at 1 ug/mL (B) lysate (35 ug protein in RIPA buffer). Detected by ...read more
Immunocytochemistry/ Immunofluorescence: STK35 Antibody [NB100-1393] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 ...read more
Immunohistochemistry-Paraffin: STK35 Antibody [NB100-1393] - Staining of Human Testis with antibody at 10 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Flow Cytometry: STK35 Antibody [NB100-1393] - Analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody ...read more
Western Blot: STK35 Antibody [NB100-1393] - Staining of Mouse with antibody at 1 ug/mL (A) and Pig with antibody at 2 ug/mL (B). Testes lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, Po, Rt, Bv, CaSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

STK35 Antibody Summary

Immunogen
Peptide with sequence ELETRMDQVTCAA corresponding to C-Terminus according to NP_543026.2.
Predicted Species
Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
STK35
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 10 ug/mL
  • Immunohistochemistry-Paraffin 10 ug/mL
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/mL
Publications
Read Publication using NB100-1393.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for STK35 Antibody

  • bA550O8.2
  • CLIK-1
  • CLIK1Serine/threonine-protein kinase 35 L1
  • CLP-36 interacting kinase
  • CLP-36-interacting kinase 1
  • EC 2.7.11
  • EC 2.7.11.1
  • PDIK1
  • PDLIM1-interacting kinase 1
  • serine threonine kinase 35 long form
  • serine/threonine kinase 35
  • serine/threonine-protein kinase 35
  • STK35L1

Background

STK35 associates with the PDZ-LIM protein CLP36 through the LIM domain and undergoes autophosphorylation in vitro. STK35 localizes predominantly to the nucleus; however, cotransfection of STK35 with CLP36 demonstrated redistribution of STK35 to the cytoplasm and into actin stress fibers, where it colocalizes with CLP36. The presence of STK35 disrupted the periodic staining pattern of CLP36 in these fibers. It is suggested that CLP36 specifically targets STK35 to actin stress fibers and that STK35 may represent a regulator of the actomyosin cytoskeleton in nonmuscle cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for STK35 Antibody (NB100-1393)(1)


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol STK35
Entrez
Uniprot