Stathmin-2/STMN2 Antibody (684433) [Unconjugated]

HAF007). For additional reference, recombinant human Stathmin-1 and recombinant human Stathmin-2 (20 ng/lane) were included. A specific band was detected for Stathmin-2/STMN2 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." > HAF007). For additional reference, recombinant human Stathmin-1 and recombinant human Stathmin-2 (20 ng/lane) were included. A specific band was detected for Stathmin-2/STMN2 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." title="Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 0.05 µg/mL of Mouse Anti-Human Stathmin-2/STMN2 Monoclonal Antibody (Catalog # MAB6930) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human Stathmin-1 and recombinant human Stathmin-2 (20 ng/lane) were included. A specific band was detected for Stathmin-2/STMN2 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." />

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 0.05 µg/mL of Mouse Anti-Human Stathmin-2/STMN2 Monoclonal Antibody (Catalog # MAB6930) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF018). A specific band was detected for Stathmin‑2/STMN2 at approximately 21-23 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." > HAF018). A specific band was detected for Stathmin‑2/STMN2 at approximately 21-23 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." title="Western blot shows lysates of Neuro‑2A mouse neuroblastoma cell line, SH‑SY5Y human neuroblastoma cell line, Mouse Brain, and Rat Brain. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse Stathmin‑2/STMN2 Monoclonal Antibody (Catalog # MAB6930) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Stathmin‑2/STMN2 at approximately 21-23 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." />

Western blot shows lysates of Neuro‑2A mouse neuroblastoma cell line, SH‑SY5Y human neuroblastoma cell line, Mouse Brain, and Rat Brain. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse Stathmin‑2/STMN2 Monoclonal Antibody (Catalog # MAB6930) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (<a class=

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." > CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." title="Stathmin-2/STMN2 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Stathmin-2/STMN2 Monoclonal Antibody (Catalog # MAB6930) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." />

Stathmin-2/STMN2 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Stathmin-2/STMN2 Monoclonal Antibody (Catalog # MAB6930) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clone: 684433
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Stathmin-2/STMN2 Antibody (684433) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Stathmin-2/STMN2
  Tyr26-Gly179
  Accession # Q93045
• Specificity: Detects human Stathmin-2/STMN2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Stathmin-1, -3, or -4 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: STMN2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 0.05 - 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Stathmin-2/STMN2 Antibody (684433) [Unconjugated]

• FLJ34868
• FLJ50995
• neuron-specific growth-associated protein
• Protein SCG10
• SCG10
• SCG10neuronal growth-associated protein (silencer element)
• SCGN10
• SCGN10stathmin-2
• Stathmin2
• Stathmin-2
• stathmin-like 2
• STMN2
• superior cervical ganglia, neural specific 10
• Superior cervical ganglion-10 protein

Background

Stathmin-2 (STMN2), also known as superior cervical ganglion 10 protein (SCG10), is a 19 kDa microtubule-destabilizing protein that is preferentially expressed in neurons. It promotes neuronal proliferation and migration as well as neurite outgrowth. Stathmin-2 contains a membrane attachment region
(aa 1‑26), a regulatory domain (aa 39-96), and an overlapping coiled-coil domain (aa 75-179). It is subject to phosphorylation of multiple serines within the regulatory domain. Within aa 26‑179, human Stathmin-2 shares 100% and 99% aa sequence identity with mouse and rat Stathmin-2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Stathmin-2/STMN2 Antibody (MAB6930)(3)

Publications using MAB6930

• Sarah Pickles, Desiree Zanetti Alepuz, Yuka Koike, Mei Yue, Jimei Tong, Pinghu Liu et al. CRISPR interference to evaluate modifiers of C9ORF72-mediated toxicity in FTD Frontiers in Cell and Developmental Biology 2023-08-07 [PMID: 37614226]
• Thornburg-Suresh, EJC;Richardson, JE;Summers, DW; The Stathmin-2 membrane targeting domain is required for axon protection and regulated degradation by DLK signaling The Journal of biological chemistry 2023-05-24 [PMID: 37236359] (Western Blot, Human)
• Foster, SL;Dutton, AJ;Yerzhan, A;March, LB;Barry, K;Seehus, CR;Huang, X;Talbot, S;Woolf, CJ; A Preliminary Study of Mild Heat Stress on Inflammasome Activation in Murine Macrophages Cells 2023-04-19 [PMID: 37190098] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: STMN2
• Uniprot:
  • Q93045()