Stathmin 1 Antibody (11E1)

Immunocytochemistry/Immunofluorescence: Stathmin 1 Antibody (11E1) [NBP2-76906] - ICC staining Stathmin 1 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Stathmin 1 monoclonal antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution.

Immunohistochemistry: Stathmin 1 Antibody (11E1) [NBP2-76906] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Stathmin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Flow Cytometry: Stathmin 1 Antibody (11E1) [NBP2-76906] - Flow cytometric analysis of Stathmin 1 was done on MG-63 cells. The cells were fixed, permeabilized and stained with Ki67 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor 488-conjugated goat anti-mouse IgG Secondary antibody at 1/500 dilution.

Immunohistochemistry: Stathmin 1 Antibody (11E1) [NBP2-76906] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Stathmin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Immunohistochemistry: Stathmin 1 Antibody (11E1) [NBP2-76906] - Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Stathmin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Product Discontinued

Stathmin 1 Antibody (11E1) Summary

• Immunogen: Synthetic peptide within Human Stathmin 1 aa 100-149 / 149. (SwissProt: P16949 Human)
• Localization: Cytoskeleton.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: STMN1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein G purified

Alternate Names for Stathmin 1 Antibody (11E1)

• C1orf215
• chromosome 1 open reading frame 215
• Lag
• Leukemia-associated phosphoprotein p18
• metablastin
• MGC138869
• Oncoprotein 18
• Op18
• OP18MGC138870
• phosphoprotein 19
• Phosphoprotein p19
• PP17
• PP19
• PR22
• prosolin
• Protein Pr22
• SMNLAP18FLJ32206
• stathmin 1
• stathmin 1/oncoprotein 18
• stathmin
• transmembrane protein C1orf215

Background

Stathmin (oncoprotein 18, op18) is a ubiquitous cytosolic phosphoprotein with various regulatory functions in cell proliferation, differentiation signaling, and activation (1). In particular, stathmin is involved in the regulation of tubulin dynamics through inhibition of microtubule formation and/or microtubule depolymerization. Stathmin interacts with soulable tubulins (alpha,beta-tubulin) resulting in the formation of T2S complex which sequesters free tubulin, impeding tubulin formation (2). Stathmin activity is regulated through phosphorylation at Ser16, Ser25, Ser38 and Ser63 by various protein kinases (e.g. MAP-kinase, p34cdc2 kinase), weakening stathmin's affinity to tubulin (3). A mutation on stathmin can lead to excess build up of mitotic spindle and with possible consequence of unregulated cell cycles seen in cancer cells (4). Stathmin is also known as the generic member of a protein family which includes neural proteins SCG10, SCLIP and RB3/RB3/RB3. All members in this family exhibits tubulin binding ability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STMN1