STAT2 Antibody (545103) [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and STAT2 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human STAT2 Monoclonal Antibody (Catalog # MAB16661) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for STAT2 at approximately 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

STAT2 was detected in immersion fixed paraffin-embedded sections of human kidney array using Mouse Anti-Human STAT2 Monoclonal Antibody (Catalog # MAB16661) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC, KO
• Clone: 545103
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

STAT2 Antibody (545103) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human STAT2
  Met1-Phe851
  Accession # P52630
• Specificity: Detects human STAT2 in direct ELISAs. In direct ELISAs, this antibody shows no cross-reactivity with rhSTAT1, 1A, 3, 4, 5A, 5B, 6, or rmSTAT2.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: STAT2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Knockout Validated
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for STAT2 Antibody (545103) [Unconjugated]

• interferon alpha induced transcriptional activator
• ISGF-3
• MGC59816
• P113
• signal transducer and activator of transcription 2
• signal transducer and activator of transcription 2, 113kD
• signal transducer and activator of transcription 2, 113kDa
• STAT113
• STAT2

Background

Signal transducer and activator of transcription 2 (STAT2) is a 98 kDa member of the transcription factor STAT family. Human STAT2 is 851 amino acids (aa) in length and contains one SH2 domain (aa 572‑667). Splicing variants produce two isoforms for human STAT2. The short isoform has substitutions corresponding to aa 621‑652 and aa 653‑851 in the long isoform, respectively. Human STAT2 shares 73% and 65% aa sequence identity with rat and mouse STAT2, in that order. STAT2 functions as a signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta ).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for STAT2 Antibody (MAB16661)(1)

Publication using MAB16661

• Zhao B, Lin J, Rong L et al. ARID1A promotes genomic stability through protecting telomere cohesion Nat Commun 9/6/2019 [PMID: 31492885]

Bioinformatics

• Gene Symbol: STAT2
• Uniprot:
  • P52630()