STAT1 Antibody (655210) [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and A431 human epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse STAT1 Monoclonal Antibody (Catalog # MAB14091) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for STAT1 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and STAT1 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human STAT1 Monoclonal Antibody (Catalog # MAB14901) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for STAT1 at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

STAT1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with IFN-alpha 1 but is not detected in STAT1 knockout (KO) HeLa cell line using Mouse Anti-Human STAT1 Monoclonal Antibody (Catalog # MAB14901) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, KO
• Clone: 655210
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

STAT1 Antibody (655210) [Unconjugated] Summary

• Immunogen: E.coli-derived recombinant human STAT1
  Ala687-Val750
  Accession # P42224
• Specificity: Detects endogenous human STAT1 in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: STAT1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for STAT1 Antibody (655210) [Unconjugated]

• CANDF7
• DKFZp686B04100
• ISGF-3
• ISGF-3,91kD
• signal transducer and activator of transcription 1, 91kDa
• signal transducer and activator of transcription-1
• STAT1
• STAT91

Background

STAT1 is a member of the STAT family of cytoplasmic transcription factors that mediate cytokine, growth factor and hormone receptor signal transduction. STAT1 is associated with type I and II interferon signaling. Phosphorylation of STAT1a at Y701 leads to dimerization and translocation to the nucleus to activate gene transcription. Human STAT1 shows 93% and 94% aa identity with mouse and rat STAT1, respectively, over the region used as an immunogen. This region is identical between isoforms STAT1a (91 kDa) and STAT1b (84 kDa).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for STAT1 Antibody (MAB14901)(1)

Publication using MAB14901

• Yamada, K;Huang, ZQ;Reily, C;Green, TJ;Suzuki, H;Novak, J;Suzuki, Y; LIF/JAK2/STAT1 Signaling Enhances Production of Galactose-Deficient IgA1 by IgA1-Producing Cell Lines Derived From Tonsils of Patients With IgA Nephropathy Kidney international reports 2024-02-01 [PMID: 38344714] (Western Blot, Human)

Bioinformatics

• Gene Symbol: STAT1
• Uniprot:
  • P42224()