STAT1 Antibody (246523) [Unconjugated]

STAT1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rat Anti-Human STAT1 Monoclonal Antibody (Catalog # MAB1490) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL014) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human STAT1 Monoclonal Antibody (Catalog # MAB1490, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram) followed by APC-conjugated Goat anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: CyTOF-ready, Flow, ICC/IF
• Clone: 246523
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

STAT1 Antibody (246523) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human STAT1
  Met1-Gln194
  Accession # P42224
• Specificity: Detects human STAT1. Detects recombinant human STAT1 transfectants but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: STAT1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for STAT1 Antibody (246523) [Unconjugated]

• CANDF7
• DKFZp686B04100
• ISGF-3
• ISGF-3,91kD
• signal transducer and activator of transcription 1, 91kDa
• signal transducer and activator of transcription-1
• STAT1
• STAT91

Background

STAT1 is a member of the STAT family of cytoplasmic transcription factors that mediate cytokine, growth factor and hormone receptor signal transduction. STAT1 is associated with type I and II interferon signaling. Phosphorylation of STAT1a at Y701 leads to dimerization and translocation to the nucleus to activate gene transcription. Human STAT1 shows 93% and 94% aa identity with mouse and rat STAT1, respectively, over the region used as an immunogen. This region is identical between isoforms STAT1a (91 kDa) and STAT1b (84 kDa).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for STAT1 Antibody (MAB1490)(2)

Publications using MAB1490

• S Li, DM Pritchard, LG Yu Galectin-3 promotes secretion of proteases that decrease epithelium integrity in human colon cancer cells Cell Death & Disease, 2023-04-13;14(4):268. 2023-04-13 [PMID: 37055381] (Western Blot, Human)
• Q Xiang, Z Yang, J Nicholas STAT and Janus kinase targeting by human herpesvirus 8 interferon regulatory factor in the suppression of type-I interferon signaling PloS Pathogens, 2022-07-01;18(7):e1010676. 2022-07-01 [PMID: 35776779] (Proximity Ligation Assay, Human)

Bioinformatics

• Gene Symbol: STAT1
• Uniprot:
  • P42224()