STAG3 Antibody

• Reactivity: Hu, Mu, Rt
• Applications: ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

STAG3 Antibody Summary

• Description: Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.
• Immunogen: Peptide with sequence C-KHYNKFYEDYGD corresponding to internal region according to Uniprot Mouse NP_058660.2.
• Epitope: C-KHYNKFYEDYGD
• Specificity: The immunizing peptide was designed based on the Mouse protein sequence with one residue of a diference from the human sequence.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: STAG3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Preliminary experiments gave an approx. 30 kDa band in human testis lysates after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 141 kDa band according to NP_058660.2. The 30 kDa band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for STAG3 Antibody

• cohesin subunit SA-3
• SCC3 homolog 3
• stromal antigen 3stromalin 3
• stromalin-3

Background

Meiosis specific component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The meiosis-specific cohesin complex probably replaces mitosis specific cohesin complex when it dissociates from chromatin during prophase I

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for STAG3 Antibody (NBP1-06084)(1)

Publication using NBP1-06084

• Storre J, Schafer A, Reichert N et al. Silencing of the meiotic genes SMC1beta and STAG3 in somatic cells by E2F6. J Biol Chem 2005-12-16 [PMID: 16236716]

Bioinformatics

• Gene Symbol: STAG3
• Entrez:
  • 10734(Human)
  • 50878(Mouse)
  • 114522(Rat)
• Uniprot:
  • NP_058660.2(Mouse)