ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Antibody [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: WB, IHC, CyTOF-ready, Flow
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 700

ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Antibody [Alexa Fluor® 700] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human α-2,8‑Sialyltransferase 8B/ST8SIA2
  Asp24-Thr375
  Accession # Q92186
• Specificity: Detects human alpha -2,8‑Sialyltransferase 8B/ST8SIA2 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunohistochemistry
  • Intracellular Staining by Flow Cytometry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Antibody [Alexa Fluor® 700]

• alpha-2,8-sialyltransferase 8B 1
• alpha-2,8-sialyltransferase 8B
• EC 2.4.99
• EC 2.4.99.-
• HsT19690
• MGC116854
• MGC116857
• sialyltransferase 8 (alpha-2, 8-sialytransferase) B
• Sialyltransferase 8B
• Sialyltransferase X
• Sialytransferase St8Sia II
• SIAT8B
• SIAT8-B
• ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2
• ST8SIA2
• ST8SiaII
• ST8SIA-II
• STX
• STXST8SiaII

Background

ST8SIA2, also known as sialyltransferase X, is mainly expressed during embryonic development (4) and shows strict preference on NCAM (6). Polysialic acid (PSA), abundant on the neural cell adhesion molecule (NCAM) during embryonic development, acts as an anti-adhesive glycan to negatively modulate the adhesive properties of NCAM (1). PSA expression decreases promptly after birth, and becomes restricted to the hippocampus, hypothalamus, and olfactory bulb, areas of the brain that require continuous cell migration and synaptic plasticity (2). Expression of PSA in cancer cells has been suggested to increase tumor invasiveness and to promote tumor growth (3). The temporal regulation of PSA is dependent on the expression of two polysialyltransferases, ST8SIA4 and ST8SIA2 (4, 5). The high degree of substrate specificity is achieved through specific enzyme-substrate recognition at both the protein sequence and glycan structure levels (6, 7). Like most glycosyltransferases, ST8SIA2 is a Golgi‑resident type II membrane protein. The activity of this enzyme has been measured with a phosphatase-coupled method (8).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.