SSX1 Antibody (OTI1E10)

Images

 
Western Blot: SSX1 Antibody (OTI1E10) [NBP2-00614] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: SSX1 Antibody (OTI1E10) [NBP2-00614] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SSX1.
Immunohistochemistry-Paraffin: SSX1 Antibody (OTI1E10) [NBP2-00614] - Staining of paraffin-embedded Human lymph node tissue using anti-SSX1 mouse monoclonal antibody.
Flow Cytometry: SSX1 Antibody (OTI1E10) [NBP2-00614] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SSX1 antibody, and then ...read more
Immunohistochemistry-Paraffin: SSX1 Antibody (OTI1E10) [NBP2-00614] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SSX1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SSX1 Antibody (OTI1E10) [NBP2-00614] - Staining of paraffin-embedded Human Kidney tissue using anti-SSX1 mouse monoclonal antibody.
Flow Cytometry: SSX1 Antibody (OTI1E10) [NBP2-00614] - Analysis of Hela cells, using anti-SSX1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: SSX1 Antibody (OTI1E10) [NBP2-00614] - Analysis of Jurkat cells, using anti-SSX1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI1E10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.64 mg/ml

Order Details

SSX1 Antibody (OTI1E10) Summary

Immunogen
Full length human recombinant protein of human SSX1 (NP_005626) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SSX1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
21.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-00614 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.64 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SSX1 Antibody (OTI1E10)

  • Cancer/testis antigen 5.1
  • CT5.1cancer/testis antigen family 5, member 1
  • MGC150425
  • protein SSX1
  • sarcoma, synovial, X-chromosome-related 1
  • SSRC
  • synovial sarcoma, X breakpoint 1MGC5162

Background

The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1, SSX2 and SSX4 genes have been involved in the t(X;18) translocation characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. The encoded hybrid proteins are probably responsible for transforming activity. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SSX1 Antibody (NBP2-00614)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, IF/IHC.


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ICC/IF
(1)
IF/IHC
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SSX1
Entrez