SSX1 Antibody (OTI1E10)

Western Blot: SSX1 Antibody (OTI1E10) [NBP2-00614] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSX1.

Immunocytochemistry/Immunofluorescence: SSX1 Antibody (OTI1E10) [NBP2-00614] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SSX1.

Immunohistochemistry-Paraffin: SSX1 Antibody (OTI1E10) [NBP2-00614] - Staining of paraffin-embedded Human lymph node tissue using anti-SSX1 mouse monoclonal antibody.

Flow Cytometry: SSX1 Antibody (OTI1E10) [NBP2-00614] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SSX1 antibody, and then analyzed by flow cytometry.

Immunohistochemistry-Paraffin: SSX1 Antibody (OTI1E10) [NBP2-00614] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SSX1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SSX1 Antibody (OTI1E10) [NBP2-00614] - Staining of paraffin-embedded Human Kidney tissue using anti-SSX1 mouse monoclonal antibody.

Flow Cytometry: SSX1 Antibody (OTI1E10) [NBP2-00614] - Analysis of Hela cells, using anti-SSX1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry: SSX1 Antibody (OTI1E10) [NBP2-00614] - Analysis of Jurkat cells, using anti-SSX1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI1E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.64 mg/ml

SSX1 Antibody (OTI1E10) Summary

• Description: Novus Biologicals Mouse SSX1 Antibody (OTI1E10) (NBP2-00614) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-SSX1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human SSX1 (NP_005626) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SSX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 21.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.64 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SSX1 Antibody (OTI1E10)

• Cancer/testis antigen 5.1
• CT5.1cancer/testis antigen family 5, member 1
• MGC150425
• protein SSX1
• sarcoma, synovial, X-chromosome-related 1
• SSRC
• synovial sarcoma, X breakpoint 1MGC5162

Background

The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1, SSX2 and SSX4 genes have been involved in the t(X;18) translocation characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. The encoded hybrid proteins are probably responsible for transforming activity. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SSX1 Antibody (NBP2-00614)(1)

Publication using NBP2-00614

• Shen JZ, Qiu Z, Wu Q et al. FBXO44 promotes DNA replication-coupled repetitive element silencing in cancer cells Cell 2020-12-18 [PMID: 33357448] (IF/IHC, ICC/IF, Human)

Bioinformatics

• Gene Symbol: SSX1
• Entrez:
  • 6756(Human)