SSH1 Antibody (1A5C8) - BSA Free

Immunocytochemistry/Immunofluorescence: SSH1 Antibody (1A5C8) [NBP2-52569] - Analysis of Hela cells using SLINGSHOT-1L mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Flow Cytometry: SSH1 Antibody (1A5C8) [NBP2-52569] - Analysis of Hela cells using SLINGSHOT-1L mouse mAb (green) and negative control (red).

ELISA: SSH1 Antibody (1A5C8) [NBP2-52569] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF, CyTOF-ready
• Clone: 1A5C8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SSH1 Antibody (1A5C8) - BSA Free Summary

• Immunogen: Synthesized peptide fragment of human SSH1 (AA: 1032-1044) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SSH1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:10000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 115.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for SSH1 Antibody (1A5C8) - BSA Free

• EC 3.1.3.16
• EC 3.1.3.48
• FLJ21928
• hSSH-1L
• KIAA1298FLJ38102
• protein phosphatase Slingshot homolog 1
• slingshot 1
• slingshot homolog 1 (Drosophila)
• SSH1L
• SSH-1L
• SSH-like protein 1

Background

SSH1, also known as slingshot, is a phosphatase that functions to activate the actin severing protein, ADF/cofilin, and influence actin dynamics. There are three members of the slingshot family, SSH1, SSH2, and SSH3. SSH1 has been shown to function downstream of alpha6beta4 integrin/Rac1 signaling in conjunction with 14-3-3 association.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SSH1