SSEA-1 Antibody (MC-480) [DyLight 755]

Product Discontinued

SSEA-1 Antibody (MC-480) [DyLight 755] Summary

• Immunogen: F9 tetracarcinoma stem cells (X-irradiated).
• Marker: Embryonic Stem Cell Marker
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: FUT4
• Purity: IgM purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: This SSEA1 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen and Paraffin.

Reactivity Notes

This antibody recognizes SSEA1 in mouse EC and ES cells but not human, but SSEA1 is expressed by some human differentiated cell types (ie: trophoblasts). Reactivity with Rat is reported by Kim et al. 2007 (PMID 17548530).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: IgM purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for SSEA-1 Antibody (MC-480) [DyLight 755]

• alpha-(1,3)-fucosyltransferase
• CD15
• EC 2.4.1
• EC 2.4.1.-
• EC 2.4.1.65
• ELAM-1 ligand fucosyltransferase
• ELFT
• FCT3AELAM ligand fucosyltransferase
• fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
• Fucosyltransferase 4
• Fucosyltransferase IV
• FUC-TIV
• fucT-IV
• FUTIV
• Galactoside 3-L-fucosyltransferase
• Lewis X
• LeX
• SSEA1
• SSEA-1
• stage-specific embryonic antigen 1

Background

SSEA1 (stage-specific mouse embryonic antigen) is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis. SSEA-1 is also known as CD15, although SSEA1 is more commonly used in a stem cell research context because SSEA-1 refers to the marker identified specifically by monoclonal antibody, clone MC-480. Clone MC-480 is now the gold standard reagent for mouse embryonic stem cells. Only mouse ESCs express SSEA1; human SSEA1 only stains cells after differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FUT4