SSEA-1 Antibody (By87a)

Product Discontinued

SSEA-1 Antibody (By87a) Summary

• Immunogen: B cell lymphoma cells
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: FUT4
• Purity: Thiophilic Chromatography

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: ultroser G can be used at 1% if the cells are not growing well. This antibody is CyTOF ready.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Thiophilic Chromatography

Alternate Names for SSEA-1 Antibody (By87a)

• alpha-(1,3)-fucosyltransferase
• CD15
• EC 2.4.1
• EC 2.4.1.-
• EC 2.4.1.65
• ELAM-1 ligand fucosyltransferase
• ELFT
• FCT3AELAM ligand fucosyltransferase
• fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
• Fucosyltransferase 4
• Fucosyltransferase IV
• FUC-TIV
• fucT-IV
• FUTIV
• Galactoside 3-L-fucosyltransferase
• Lewis X
• LeX
• SSEA1
• SSEA-1
• stage-specific embryonic antigen 1

Background

SSEA1 (stage-specific mouse embryonic antigen) is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis. SSEA-1 is also known as CD15, although SSEA1 is more commonly used in a stem cell research context because SSEA-1 refers to the marker identified specifically by monoclonal antibody, clone MC-480. Clone MC-480 is now the gold standard reagent for mouse embryonic stem cells. Only mouse ESCs express SSEA1; human SSEA1 only stains cells after differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FUT4