SRPRB Antibody (OTI2D4) - Azide and BSA Free

Western Blot: SRPRB Antibody (OTI2D4) - Azide and BSA Free [NBP2-74351] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SRPRB.

Immunohistochemistry: SRPRB Antibody (OTI2D4) - Azide and BSA Free [NBP2-74351] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Western Blot: SRPRB Antibody (OTI2D4) - Azide and BSA Free [NBP2-74351] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry: SRPRB Antibody (OTI2D4) - Azide and BSA Free [NBP2-74351] - Analysis of Adenocarcinoma of Human colon tissue . (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: SRPRB Antibody (OTI2D4) - Azide and BSA Free [NBP2-74351] - Analysis of Carcinoma of Human bladder tissue using. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: SRPRB Antibody (OTI2D4) - Azide and BSA Free [NBP2-74351] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: SRPRB Antibody (OTI2D4) - Azide and BSA Free [NBP2-74351] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI2D4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

SRPRB Antibody (OTI2D4) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse SRPRB Antibody (OTI2D4) - Azide and BSA Free (NBP2-45958) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human SRPRB(NP_067026) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SRPRB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 29.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for SRPRB Antibody (OTI2D4) - Azide and BSA Free

• APMCF1
• Protein APMCF1
• signal recognition particle receptor subunit beta
• signal recognition particle receptor, B subunit
• signal recognition particle receptor, beta subunit
• SR-beta

Background

SRPRB is encoded by this gene has similarity to mouse protein which is a subunit of the signal recognition particle receptor (SR). This subunit is a transmembrane GTPase belonging to the GTPase superfamily. It anchors alpha subunit, a peripheral membrane GTPase, to the ER membrane. SR is required for the cotranslational targeting of both secretory and membrane proteins to the ER membrane. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SRPRB