SRPK2 Knockout HeLa Cell Lysate

Product Discontinued

SRPK2 Knockout HeLa Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,5 bp deletion in exon 3
• Gene: SRPK2

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout HeLa Cell

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for SRPK2 Knockout HeLa Cell Lysate

• EC 2.7.11.1
• FLJ36101
• H_RG152G17.1a
• H_RG152G17.1b
• serine kinase SRPK2
• Serine/arginine-rich protein-specific kinase 2
• serine/arginine-rich splicing factor kinase 2
• serine/threonine-protein kinase SRPK2
• SFRS protein kinase 2SFRSK2
• SFRSK2
• SR protein kinase 2
• SRPK2
• SR-protein-specific kinase 2
• SRSF protein kinase 2
• WUGSC:H_RG152G17.1a

Background

Serine/arginine rich protein-specific kinase 2 (SRPK2) phosphorylates RS domain-containing proteins, such as SFRS1 and SFRS2 on serine residues. SRPK2 plays a role in spliceosome assembly and in mediating the trafficking of splicing factors. SRPK2 also appears to mediate HBV core protein phosphorylation which is a prerequisite for pregenomic RNA encapsidation into viral capsids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: SRPK2