Spinesin Antibody (376620)

• Reactivity: Hu
• Applications: WB, IP
• Clone: 376620
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Spinesin Antibody (376620) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Spinesin
  Tyr27-Leu457 (Phe369Leu)
  Accession # Q0P514
• Specificity: Detects human Spinesin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TMPRSS5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Spinesin Antibody (376620)

• EC 3.4.21
• EC 3.4.21.-
• EC 3.4.21.4
• MGC141886
• MGC148044
• Spinesin
• TMPRSS5
• transmembrane protease serine 5
• transmembrane protease, serine 5

Background

Spinesin, encoded by the TMPRSS5 gene, is a member of type II transmembrane serine proteases (TTSPs) (1). Human Spinesin contains the following structural domains: a short N‑terminal cytoplasmic tail (amino acids 1‑49), a transmembrane domain (amino acids 50‑70), a stem region and a serine protease domain (amino acids 71‑457) (2). The domain structure of Spinesin is common to other TTSPs, many of which have additional domains. The stem region of Spinesin contains a scavenger receptor-like domain. The ectodomain of human Spinesin (amino acids 71‑457) was expressed and purified as a single chain pro-enzyme. The deduced amino acid sequence contains a Leu instead of a Phe residue at position 369; the former is identical to the mouse protein (3, 4). The amino acid sequence of human Spinesin is 94%, 82%, 81% and 79% identical to that of chimpanzee, canine, rat and mouse.

1. Hooper, J.D. et al. (2001) J. Biol. Chem. 276:857.
2. Yamaguchi, Y. et al. (2002) J. Biol. Chem. 277:6806.
3. Carninci, P. et al. (2000) Genome Res. 10:1617.
4. Shibata, K. et al. (2000) Genome Res. 10:1757.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Spinesin Antibody (MAB24951)(1)

Publication using MAB24951

• Hongge Wang, Matthew Davison, Kathryn Wang, Tai‐He Xia, Martin Kramer, Katherine Call et al. Transmembrane protease serine 5: a novel Schwann cell plasma marker for CMT1A Annals of Clinical and Translational Neurology 2020-01-01 [PMID: 31833243]

Bioinformatics

• Gene Symbol: TMPRSS5
• Uniprot:
  • Q0P514()