SPFH2 Antibody

Western Blot: SPFH2 Antibody [21320002] - Samples: Lane 1, Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil, Target weight [kDa]: 38, 17 (splice variants) Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.

Immunohistochemistry: SPFH2 Antibody [21320002] - Strong cytoplasmic staining, most likely located in the endoplasmatic reticulum, was observed in a majority of normal and malignant tissues. Many cases of non-Hodgkin's lymphoma were negative. Image and statement courtesy of the Human Protein Atlas (HPA).

ELISA: SPFH2 Antibody [21320002]

Product Discontinued

SPFH2 Antibody Summary

• Immunogen: This antibody is specific for the C Terminus Region of the target protein.
• Specificity: This product is specific for Human SPFH2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ERLIN2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:5000-1:20000
• Application Notes: This antibody is useful in ELISA, Immunohistochemistry-Paraffin and Western Blot.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.

Alternate Names for SPFH2 Antibody

• C8orf2SPFH domain-containing protein 2
• Endoplasmic reticulum lipid raft-associated protein 2
• ER lipid raft associated 2
• Erlin-2
• MGC87072
• NET32
• SPFH domain family, member 2
• SPFH2chromosome 8 open reading frame 2
• Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2

Background

SPFH2 is a component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). SPFH2 is also involved in ITPR1 degradation by the ERAD pathway

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SPFH2 Antibody (21320002)(2)

Publications using 21320002

• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.

Bioinformatics

• Gene Symbol: ERLIN2
• Uniprot:
  • NM_007175(Human)