SPAM1 Antibody - BSA Free

Western Blot: SPAM1 Antibody - BSA Free [NBP3-45852] - PC-3 cells were subjected to SDS PAGE followed by western blot with (SPAM1 antibody) at dilution of 1:1000

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SPAM1 Antibody - BSA Free Summary

• Immunogen: sperm adhesion molecule 1(PH-20 hyaluronidase, zona pellucida binding). (Uniprot# P38567)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPAM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500-1:2000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SPAM1 Antibody - BSA Free

• EC 3.2.1.35
• HYA1
• HYAL1
• HYAL3
• HYAL5
• hyal-PH20
• hyaluronidase PH-20
• Hyaluronoglucosaminidase PH-20
• MGC26532
• PH20
• PH-20
• SPAG15
• SPAM1
• sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)
• Sperm adhesion molecule 1
• Sperm surface protein PH-20

Background

Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple protein isoforms are encoded by transcript variants of this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPAM1