SPAG1 Antibody (10G1/2) [Alexa Fluor™ Plus 594]

• Reactivity: Hu
• Applications: WB, ICC/IF, IP
• Clone: 10G1/2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 594

SPAG1 Antibody (10G1/2) [Alexa Fluor™ Plus 594] Summary

• Immunogen: Synthetic peptide SPA-3 (sequence designed to avoid TPR motifs - KTAPFNKEKERRKIEIQEVNE)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: SPAG1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for SPAG1 Antibody (10G1/2) [Alexa Fluor™ Plus 594]

• FLJ32920
• HSD-3.8sperm-associated antigen 1
• Infertility-related sperm protein Spag-1
• SP75
• sperm associated antigen 1
• tetratricopeptide repeat-containing protein
• TPIS
• TPR-containing protein involved in spermatogenesis

Background

The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm agglutinating antibodies from an infertile woman. Furthermore, immunization of female rats with the recombinant human protein reduced fertility. This protein localizes to the plasma membrane of germ cells in the testis and to the post-acrosomal plasma membrane of mature spermatozoa. Recombinant polypeptide binds GTP and exhibits GTPase activity. Thus, this protein may regulate GTP signal transduction pathways involved in spermatogenesis and fertilization. Two transcript variants of this gene encode the same protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPAG1