SP4 Antibody

Western Blot: SP4 Antibody [NBP2-97665] - Anti-SP4 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 82 kDa. Observed band size: 97 kDa

Immunocytochemistry/Immunofluorescence: SP4 Antibody [NBP2-97665] - Immunofluorescence staining of SP4 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SP4 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.

Immunohistochemistry-Paraffin: SP4 Antibody [NBP2-97665] - Immunochemical staining of human SP4 in human spleen with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: SP4 Antibody [NBP2-97665] - Immunochemical staining of human SP4 in human lymph node with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.

Product Discontinued

SP4 Antibody Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human SP4 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SP4
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.0)
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for SP4 Antibody

• MGC130008
• MGC130009
• Sp4 transcription factor
• SPR-1HF1B
• transcription factor Sp4

Background

SP4 binds to GT and GC boxes promoters elements. Probable transcriptional activator

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SP4