Porcine SP-B/Surfactant Protein B ELISA Kit (Colorimetric)

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Standard Curve Reference.
Samples were spiked with high concentrations of Porcine SP-B/Surfactant Protein B, and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Product Details

Summary
Reactivity PoSpecies Glossary
Applications ELISA
Standard Curve Range
3.13 - 200 ng/mL
Sensitivity
1.88 ng/mL

Order Details

Porcine SP-B/Surfactant Protein B ELISA Kit (Colorimetric) Summary

Description
Assay Length: 3 h 30 min
Standard Curve Range
3.13 - 200 ng/mL
Sensitivity
1.88 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 10
Intra-Assay
CV% < 10
Spike Recovery
80 - 120%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
SFTPB

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Certificate of Analysis
  3. Concentrated Biotinylated Detection Ab (100x)
  4. Concentrated HRP Conjugate (100x)
  5. Concentrated Wash Buffer (25x)
  6. HRP Conjugate Diluent
  7. Micro ELISA Plate (Dismountable)
  8. Plate Sealer
  9. Product Description
  10. Reference Standard & Sample Diluent
  11. Reference Standard
  12. Stop Solution
  13. Substrate Reagent

Alternate Names for Porcine SP-B/Surfactant Protein B ELISA Kit (Colorimetric)

  • 18 kDa pulmonary-surfactant protein
  • 6 kDa protein
  • PSP-B
  • pulmonary surfactant-associated protein B
  • Pulmonary surfactant-associated proteolipid SPL(Phe)
  • SFTB3
  • SFTP3
  • SFTPB
  • SMDP1
  • SP-B surfactant, pulmonary-associated protein B
  • SP-B
  • surfactant protein B

Background

The SFTPB gene encodes the pulmonary-associated surfactant B protein (SPB), an amphipathic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a lipid-rich material that prevents lung collapse by lowering surface tension at the air-liquid interface in the alveoli of lung. SPB enhances the rate of spreading and increases the stability of surfactant monolayers in vitro. Surfactant is composed of phospholipids and other surfactant-associated proteins (Clark et al., 1995 [PubMed 7644495]). See also SFTPA1 (MIM 178630), SFTPC (MIM 178620), and SFTPD (MIM 178635).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for SP-B/Surfactant Protein B ELISA Kit (NBP3-49943). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Bioinformatics

Gene Symbol SFTPB