SOX3 Antibody

Western Blot: SOX3 Antibody [NBP1-52043] - NBP1-52043 (0.5ug/ml) staining of Human Cerebral Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: SOX3 Antibody [NBP1-52043] - (3.8ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

SOX3 Antibody Summary

• Immunogen: Peptide with sequence C-DAASPLPGGRLHGVH corresponding to C-Terminus according to NP_005625.2.
• Epitope: C-DAASPLPGGRLHGVH
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SOX3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.5 - 1.5 ug/ml
• Application Notes: WB: Approx. 42 kDa band observed in human brain (cerebellum, cerebral cortex and frontal cortex) lysates (calculated MW of 45.2 kDa band according to NP_005625.2). IHC-P: Human Cerebrl cortex shows nuclear staining at verious intensities along the neuronal cells.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SOX3 Antibody

• GHDX
• MRGH
• panhypopituitarism
• PHP
• PHPX
• SOX3
• SOXB
• SRY (sex determining region Y)-box 3
• transcription factor SOX-3

Background

This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. Mutations in this gene have been associated with X-linked mental retardation with growth hormone deficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SOX3 Antibody (NBP1-52043)(1)

Publication using NBP1-52043

• Mojsin M, Stevanovic M. PBX1 and MEIS1 up-regulate SOX3 gene expression by direct interaction with a consensus binding site within the basal promoter region. Biochem J 2010-01-01 [PMID: 19799567]

Bioinformatics

• Gene Symbol: SOX3
• Entrez:
  • 6658(Human)
• Uniprot:
  • NP_005625.2(Human)