SOX3 Antibody

Images

 
Western Blot: SOX3 Antibody [NBP1-52043] - NBP1-52043 (0.5ug/ml) staining of Human Cerebral Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: SOX3 Antibody [NBP1-52043] - (3.8ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

SOX3 Antibody Summary

Immunogen
Peptide with sequence C-DAASPLPGGRLHGVH corresponding to C-Terminus according to NP_005625.2.
Epitope
C-DAASPLPGGRLHGVH
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SOX3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1.5 ug/ml
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: Approx. 42 kDa band observed in human brain (cerebellum, cerebral cortex and frontal cortex) lysates (calculated MW of 45.2 kDa band according to NP_005625.2). IHC-P: Human Cerebrl cortex shows nuclear staining at verious intensities along the neuronal cells.
Publications
Read Publication using NBP1-52043.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SOX3 Antibody

  • GHDX
  • MRGH
  • panhypopituitarism
  • PHP
  • PHPX
  • SOX3
  • SOXB
  • SRY (sex determining region Y)-box 3
  • transcription factor SOX-3

Background

This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. Mutations in this gene have been associated with X-linked mental retardation with growth hormone deficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SOX3 Antibody (NBP1-52043)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SOX3
Entrez
Uniprot