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SOX3 Antibodies

We offer SOX3 Antibodies for use in common research applications: ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot. Each SOX3 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our SOX3 Antibodies can be used in a variety of model species: Human. Use the list below to choose the SOX3 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our SOX3 polyclonal antibodies and browse our SOX3 monoclonal antibody catalog.

Alternate Names for SOX3 Antibodies

anti-SOX3 antibody, anti-GHDX antibody, anti-MRGH antibody, anti-panhypopituitarism antibody, anti-PHP antibody, anti-PHPX antibody, anti-SOXB antibody, anti-SRY (sex determining region Y)-box 3 antibody, anti-transcription factor SOX-3 antibody

6 Results for "sox3" in Primary Antibodies

AF2569

SOX3 Antibody [Unconjugated]

(13 Publications)

100 μg

AF2569

SOX3 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line differentiation with retinoic acid using Goat Anti-Human SOX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2569) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green, upper panel; NL003) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

SOX3 was detected in immersion fixed U‑251 MG human glioblastoma cells (positive) and absent in HDLM‑2 human Hodgkin’s lymphoma cells (negative) using Goat Anti-Human SOX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2569) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Transcription is unaffected but protein is cleared from mutant cells.A) SOX3 protein is present in every WT cell (NEO−) of the 13.5 dpc telencephalic ventricular zone but virtually absent from equivalent tissue derived from Sox3-26ala cells (NEO+). B) Comparison of SOX3 immunostaining on Sox3-null cells (from a 14.5 dpc +/− embryo) and Sox3-26ala expressing cells (from a Sox3-26ala WT chimera) confirming that the antibody is SOX3-specific and that the Sox3-26ala expressing cells exhibit a low level of residual nuclear protein. C) WT, Neo, Sox3-26ala and Sox3-null ES cells were differentiated for 5 days in CDM as multi-cellular bodies. Rare SOX3 positive cells were detected in Sox3-26ala CDMs while the majority of cells had low SOX3 protein levels in comparison to neighbouring WT CDM bodies processed on the same slide. D–E) WT, Neo, Sox3-26ala and Sox3-null ES cells were grown in N2B27 for 4 days to form neural progenitors. Western blotting for SOX3 reveals a dramatic reduction of protein in Sox3-26ala cells (D); 3 and 30 minute exposures are shown. E) Transcript levels of Sox3 are not affected in Sox3-26ala cells as determined by qPCR. Three experimental replicates are shown. Data was normalised to Sox3 levels inSox3-Neo control cells and error bars represent SEM. F) ISH confirms that Sox3 transcript is present at comparable levels in ventricular zone cells at 13.5 dpc derived from both WT (Neo−) and Sox3-26ala (Neo+) cells. ISH performed on adjacent 10 µm coronal sections of 13.5 dpc chimeric telencephalon. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23505376), licensed under a CC-BY license. Not internally tested by R&D Systems.

Transcription is unaffected but protein is cleared from mutant cells.A) SOX3 protein is present in every WT cell (NEO−) of the 13.5 dpc telencephalic ventricular zone but virtually absent from equivalent tissue derived from Sox3-26ala cells (NEO+). B) Comparison of SOX3 immunostaining on Sox3-null cells (from a 14.5 dpc +/− embryo) and Sox3-26ala expressing cells (from a Sox3-26ala WT chimera) confirming that the antibody is SOX3-specific and that the Sox3-26ala expressing cells exhibit a low level of residual nuclear protein. C) WT, Neo, Sox3-26ala and Sox3-null ES cells were differentiated for 5 days in CDM as multi-cellular bodies. Rare SOX3 positive cells were detected in Sox3-26ala CDMs while the majority of cells had low SOX3 protein levels in comparison to neighbouring WT CDM bodies processed on the same slide. D–E) WT, Neo, Sox3-26ala and Sox3-null ES cells were grown in N2B27 for 4 days to form neural progenitors. Western blotting for SOX3 reveals a dramatic reduction of protein in Sox3-26ala cells (D); 3 and 30 minute exposures are shown. E) Transcript levels of Sox3 are not affected in Sox3-26ala cells as determined by qPCR. Three experimental replicates are shown. Data was normalised to Sox3 levels inSox3-Neo control cells and error bars represent SEM. F) ISH confirms that Sox3 transcript is present at comparable levels in ventricular zone cells at 13.5 dpc derived from both WT (Neo−) and Sox3-26ala (Neo+) cells. ISH performed on adjacent 10 µm coronal sections of 13.5 dpc chimeric telencephalon. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23505376), licensed under a CC-BY license. Not internally tested by R&D Systems.

SOX3-26ala from mouse and human retains transactivation activity.A) COS-7 cells were transfected with pcDNA3.1 expression vector containing either mouse Sox3, human SOX3, mouse Sox3-26ala, human SOX3-26ala or an empty vector control. Values represent mean normalised luciferase values plus standard deviation of four independent experiments measured 48 hours after transfection. Student's T-tests (two tailed, unequal variance) of SOX3-26ala from human or mouse compared to empty vector control show a statistically significant increase in luciferase activity. B) Nuclear protein lysates prepared from duplicate plates 48 hours after transfection show that less SOX3 is detected in the nucleus of cells expressing both mouse and human SOX3-26ala. pcDNA3.1-EGFP transfected cells were used as a control and prepared for both nuclear protein and whole cell extracts (WCE). Blotting for Histone H3, indicates equal loading and blotting for alpha -Tubulin shows an absence of cytoplasmic contamination in nuclear preparations. Transfection efficiency was determined by co-transfecting EGFP and counting positive cells prior to harvesting and found to be equal for all plasmids. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23505376), licensed under a CC-BY license. Not internally tested by R&D Systems.

Transcription is unaffected but protein is cleared from mutant cells.A) SOX3 protein is present in every WT cell (NEO−) of the 13.5 dpc telencephalic ventricular zone but virtually absent from equivalent tissue derived from Sox3-26ala cells (NEO+). B) Comparison of SOX3 immunostaining on Sox3-null cells (from a 14.5 dpc +/− embryo) and Sox3-26ala expressing cells (from a Sox3-26ala WT chimera) confirming that the antibody is SOX3-specific and that the Sox3-26ala expressing cells exhibit a low level of residual nuclear protein. C) WT, Neo, Sox3-26ala and Sox3-null ES cells were differentiated for 5 days in CDM as multi-cellular bodies. Rare SOX3 positive cells were detected in Sox3-26ala CDMs while the majority of cells had low SOX3 protein levels in comparison to neighbouring WT CDM bodies processed on the same slide. D–E) WT, Neo, Sox3-26ala and Sox3-null ES cells were grown in N2B27 for 4 days to form neural progenitors. Western blotting for SOX3 reveals a dramatic reduction of protein in Sox3-26ala cells (D); 3 and 30 minute exposures are shown. E) Transcript levels of Sox3 are not affected in Sox3-26ala cells as determined by qPCR. Three experimental replicates are shown. Data was normalised to Sox3 levels inSox3-Neo control cells and error bars represent SEM. F) ISH confirms that Sox3 transcript is present at comparable levels in ventricular zone cells at 13.5 dpc derived from both WT (Neo−) and Sox3-26ala (Neo+) cells. ISH performed on adjacent 10 µm coronal sections of 13.5 dpc chimeric telencephalon. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23505376), licensed under a CC-BY license. Not internally tested by R&D Systems.

Transcription is unaffected but protein is cleared from mutant cells.A) SOX3 protein is present in every WT cell (NEO−) of the 13.5 dpc telencephalic ventricular zone but virtually absent from equivalent tissue derived from Sox3-26ala cells (NEO+). B) Comparison of SOX3 immunostaining on Sox3-null cells (from a 14.5 dpc +/− embryo) and Sox3-26ala expressing cells (from a Sox3-26ala WT chimera) confirming that the antibody is SOX3-specific and that the Sox3-26ala expressing cells exhibit a low level of residual nuclear protein. C) WT, Neo, Sox3-26ala and Sox3-null ES cells were differentiated for 5 days in CDM as multi-cellular bodies. Rare SOX3 positive cells were detected in Sox3-26ala CDMs while the majority of cells had low SOX3 protein levels in comparison to neighbouring WT CDM bodies processed on the same slide. D–E) WT, Neo, Sox3-26ala and Sox3-null ES cells were grown in N2B27 for 4 days to form neural progenitors. Western blotting for SOX3 reveals a dramatic reduction of protein in Sox3-26ala cells (D); 3 and 30 minute exposures are shown. E) Transcript levels of Sox3 are not affected in Sox3-26ala cells as determined by qPCR. Three experimental replicates are shown. Data was normalised to Sox3 levels inSox3-Neo control cells and error bars represent SEM. F) ISH confirms that Sox3 transcript is present at comparable levels in ventricular zone cells at 13.5 dpc derived from both WT (Neo−) and Sox3-26ala (Neo+) cells. ISH performed on adjacent 10 µm coronal sections of 13.5 dpc chimeric telencephalon. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23505376), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2569-SP

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NBP1-52043

SOX3 Antibody

(1 Publication)

0.1 mg

NBP1-52043

Western Blot: SOX3 Antibody [NBP1-52043] - NBP1-52043 (0.5ug/ml) staining of Human Cerebral Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: SOX3 Antibody [NBP1-52043] - (3.8ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Reactivity:

Hu, Mu, Rt, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Ca

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Alt Catalog#

IMG-31276

Alt Catalog# IMG-31276

Conjugate:

Unconjugated

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NBP2-57856

SOX3 Antibody - BSA Free

100 ul

NBP2-57856

Immunocytochemistry/Immunofluorescence: SOX3 Antibody [NBP2-57856] - Staining of human cell line AF22 shows localization to nucleoplasm.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-57856-25ul

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NBP2-59060

SOX3 Antibody (CL4725) - BSA Free

100 ul

NBP2-59060

Western Blot: SOX3 Antibody (CL4725) [NBP2-59060] - Analysis in human cell line NTERA-2.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-44124AF350
Alexa Fluor 405	NBP3-44124AF405
Alexa Fluor 488	NBP3-44124AF488
Alexa Fluor 532	NBP3-44124AF532
Alexa Fluor 594	NBP3-44124AF594
Alexa Fluor 647	NBP3-44124AF647
Alexa Fluor 700	NBP3-44124AF700
Alexa Fluor 750	NBP3-44124AF750
Allophycocyanin	NBP3-44124APC
Biotin	NBP3-44124B
CoraFluor 1	NBP3-44124CL1
DyLight 350	NBP3-44124UV
DyLight 405	NBP3-44124V
DyLight 488	NBP3-44124G
DyLight 550	NBP3-44124R
DyLight 594	NBP3-44124DL594
DyLight 650	NBP3-44124C
DyLight 680	NBP3-44124FR
DyLight 755	NBP3-44124IR
FITC	NBP3-44124F
HRP	NBP3-44124H
Janelia Fluor 525	NBP3-44124JF525
Janelia Fluor 549	NBP3-44124JF549
Janelia Fluor 646	NBP3-44124JF646
Janelia Fluor 669	NBP3-44124JF669
PE	NBP3-44124PE
PerCP	NBP3-44124PCP
mFluor Violet 450 SE	NBP3-44124MFV450
mFluor Violet 500 SE	NBP3-44124MFV500
mFluor Violet 610 SE	NBP3-44124MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-44124
BSA Free	NBP2-59060-25ul

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NBP2-55569

SOX3 Antibody - BSA Free

100 ul

NBP2-55569

Immunocytochemistry/Immunofluorescence: SOX3 Antibody [NBP2-55569] - Staining of human cell line AF22 shows localization to nucleoplasm.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-55569-25ul

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H00006658-M04

SOX3 Antibody (1F2) - Azide and BSA Free

0.1 mg

H00006658-M04

Western Blot: SOX3 Antibody (1F2) [H00006658-M04] - Analysis of SOX3 expression in A-431 (Cat # L015V1).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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