SOX22 Antibody (1079017) [Unconjugated]

SOX12 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line (Positive) and SH‑SY5Y human neuroblastoma cell line (Positive) using Mouse Anti-Human SOX12 Monoclonal Antibody (Catalog # MAB11733) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

SOX12 was detected in immersion fixed paraffin-embedded sections of human breast using Mouse Anti-Human SOX12 Monoclonal Antibody (Catalog # MAB11733) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC, ICC/IF
• Clone: 1079017
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

SOX22 Antibody (1079017) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human SOX12
  Accession # O15370
• Specificity: Detects recombinant human SOX12 protein in Direct ELISA.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 3-25 ug/mL
  • Immunohistochemistry 3-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution Instructions: Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOX22 Antibody (1079017) [Unconjugated]

• Protein SOX-22
• SOX12
• SOX-22 protein
• SOX22
• SOX22transcription factor SOX-12
• SRY (sex determining region Y)-box 12
• SRY (sex determining region Y)-box 22
• SRY-related HMG-box gene 22

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.