Sodium Potassium ATPase Beta 1 Antibody

Western Blot: Sodium Potassium ATPase Beta 1 Antibody [NBP1-51987] - Staining of Rat Retina lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP1-51987] - Staining of Human Small Intestine. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Sodium Potassium ATPase Beta 1 Antibody Summary

• Immunogen: Peptide with sequence C-KTEISFRPNDPKSYE corresponding to internal region according to NP_001668.1, NP_001001787.1.
• Epitope: C-KTEISFRPNDPKSYE
• Marker: Plasma Membrane Marker
• Specificity: This antibody is expected to recognize both reported isoforms (NP_001668.1; NP_001001787.1).
• Predicted Species: Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ATP1B1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.1 - 0.3 ug/mL
• Application Notes: WB: Approx. 48 kDa band observed in rat retina lysates and 50 kDa band in mouse and rat kidney lysates is reported in scientific literature (PMID: 20130120).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Sodium Potassium ATPase Beta 1 Antibody

• adenosinetriphosphatase
• ATP1B
• ATP1B1
• ATPase, Na+/K+ transporting, beta 1 polypeptide
• Beta 1-subunit of Na(+)
• K(+)-ATPase
• MGC1798
• Na, K-ATPase beta-1 polypeptide
• Sodium Potassium ATPase Beta 1
• sodium/potassium-dependent ATPase beta-1 subunit
• Sodium/potassium-dependent ATPase subunit beta-1
• sodium/potassium-transporting ATPase beta-1 chain
• sodium/potassium-transporting ATPase subunit beta-1

Background

Na,K-ATPase is an integral membrane protein complex that hydrolyzes ATP to maintain the transmembrane gradients of Na+ and K+ found in most mammalian cells. The enzyme is comprised of an alpha and beta subunit. The alpha-polypeptide has been shown to be the catalytically active subunit, whereas the beta-polypeptide is glycosylated and appears to be necessary for the assembly and transport of the sodium pump to the plasma membrane.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sodium Potassium ATPase Beta 1 Antibody (NBP1-51987)(3)

Publications using NBP1-51987

• van der Veen EL, Giesen D, Pot-de Jong L et al. 89 Zr-pembrolizumab biodistribution is influenced by PD-1-mediated uptake in lymphoid organs Journal for Immunotherapy of Cancer 2020-10-01 [PMID: 33020241]
• Herman MB, Rajkhowa T, Cutuli F et al. Regulation of renal proximal tubule Na-K-ATPase by prostaglandins. Am J Physiol Renal Physiol 2010-05-01 [PMID: 20130120]
• Ushkaryov YuA, Monastyrskaya GS, Broude NE et al. Human Na+,K+-ATPase genes. Beta-subunit gene family contains at least one gene and one pseudogene FEBS Lett 1989-11-01 [PMID: 2555225]

Bioinformatics

• Gene Symbol: ATP1B1
• Entrez:
  • 481(Human)
• Uniprot:
  • NP_001668.1(Human)