Sodium Potassium ATPase Alpha 2 Antibody

Product Discontinued

Sodium Potassium ATPase Alpha 2 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids C-terminus of Human Na+/K+-ATPase alpha2.
• Marker: Plasma Membrane Marker
• Specificity: Na+/K+-ATPase alpha2 (R1007) detects endogenous levels of Na+/K+-ATPase alpha2 protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATP1A2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000-1:5000
  • Western Blot 1:500-1:1000
• Application Notes: Western blot analysis of Na+/K+-ATPase alpha2 (R1007) antibody in extracts from Jurkat cells. Detects a band at molecular weight: 112kDa
• Theoretical MW: 112 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This antibody is reactive against Human, Mouse, Rat

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Sodium Potassium ATPase Alpha 2 Antibody

• alpha-A(+) catalytic polypeptide
• ATP1A2
• ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide
• ATPase, Na+/K+ transporting, alpha 2 polypeptide
• EC 3.6.3
• EC 3.6.3.9
• KIAA0778
• MGC59864
• MHP2
• migraine, hemiplegic 2
• Na(+)/K(+) ATPase alpha-2 subunit
• Na+/K+ ATPase, alpha-B polypeptide
• Sodium Potassium ATPase Alpha 2
• Sodium pump subunit alpha-2
• sodium/potassium-transporting ATPase alpha-2 chain
• sodium/potassium-transporting ATPase subunit alpha-2
• sodium-potassium ATPase

Background

The ubiquitously expressed sodium/potassium-ATPase exists as a oligomeric plasma membrane complex that couples the hydrolysis of one molecule of ATP to the importation of three Na+ ions and two K+ ions against their respective electrochemical gradients. As a member of the P-type family of ion motifs, sodium/potassium-ATPase plays a critical role in maintaining cellular volume, resting membrane potential and Na+-coupled solute transport. Multiple isoforms of three subunits, alpha, beta and gamma, comprise to form the sodium/potassium-ATPase oligomer. The 113 kDa alpha subunit contains the binding sites for ATP and the cations. With a molecular weight between 40 and 60 kDa, the glycosylated beta subunit ensures correct folding and membrane insertion of the alpha subunits. The small 6 kDa gamma subunit co-localizes with the alpha subunit in nephron segments, where it increases the affinity of sodium/potassium-ATPase for ATP. The beta subunit, but not the gamma subunit, is essential for normal activity of sodium/potassium-ATPase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sodium Potassium ATPase Alpha 2 Antibody (NBP1-00937)(2)

Publications using NBP1-00937

• Tadi M, Allaman I, Lengacher S et al. Learning-Induced Gene Expression in the Hippocampus Reveals a Role of Neuron -Astrocyte Metabolic Coupling in Long Term Memory. PLoS ONE. 2015-10-30 [PMID: 26513352] (WB, Mouse)
• Kong HK, Wong MH, Chan HM, Lo SC. Chronic Exposure of Adult Rats to Low Doses of Methylmercury Induced a State of Metabolic Deficit in the Somatosensory Cortex. J Proteome Res. 2013-08-28 [PMID: 23984759] (WB, Rat)

Bioinformatics

• Gene Symbol: ATP1A2
• Entrez:
  • 477(Human)
  • 24212(Rat)
• Uniprot:
  • P50993(Human)