Sodium Potassium ATPase Alpha 1 [p Tyr10] Antibody

Images

 
Western Blot: Sodium Potassium ATPase Alpha 1 [p Tyr10] Antibody [NBP2-22107] - Analysis of Na+/K+-ATPase alpha 1 (Y10) pAb in extracts from 293 PMA 125ng/ml 30' cells.
Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 [p Tyr10] Antibody [NBP2-22107] - Analyzes of paraffin-embedded human breast carcinoma tissue at 1:100.
Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 [p Tyr10] Antibody [NBP2-22107] - Analyzes of Na+/K+-ATPase alpha 1 (Y10) pAb in paraffin-embedded human breast carcinoma tissue.

Order Details


    • Catalog Number
      NBP2-22107
    • Availability
      Product Discontinued

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Sodium Potassium ATPase Alpha 1 [p Tyr10] Antibody Summary

Immunogen
This antibody detects Sodium Potassium ATPase Alpha 1 at phospho Tyrosine 10.
Modification
p Tyr10
Marker
Plasma Membrane Marker
Specificity
Na+/K+-ATPase alpha 1 (Y10) pAb detects endogenous levels of A Na+/K+-ATPase alpha 1 protein.
Clonality
Polyclonal
Host
Rabbit
Gene
ATP1A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:10-1:500
Theoretical MW
112 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Sodium Potassium ATPase Alpha 1 [p Tyr10] Antibody

  • ATPase, Na+/K+ transporting, alpha 1 polypeptide
  • EC 3.6.3
  • EC 3.6.3.9
  • K-ATPase alpha-1 subunit
  • K-ATPase catalytic subunit alpha-A protein
  • MGC3285
  • MGC51750
  • Na
  • Na(+)/K(+) ATPase alpha-1 subunit
  • Na, K-ATPase, alpha-A catalytic polypeptide
  • Na+, K+ ATPase alpha subunit
  • Na+/K+ ATPase 1
  • sodium pump 1
  • Sodium pump subunit alpha-1
  • sodium/potassium-transporting ATPase subunit alpha-1
  • sodium-potassium-ATPase, alpha 1 polypeptide

Background

The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP dependent transport of sodium and potassium across the cell membrane. This membrane bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium / potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families.The different isoforms of the sodium / potassium ATPase exhibit tissue specific and developmental patterns of expression. The alpha 1 and beta mRNAs are present in all cell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell specific expression. The alpha subunit has been found in kidney, brain, heart, and to a lesser extent liver, skeletal and smooth muscle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Sodium Potassium ATPase Alpha 1 Antibody (NBP2-22107). (Showing 1 - 1 of 1 FAQ).

  1. I am looking forward to pick up a plasma membrane marker. I have gastric cancer cell line and I am preparing PM out of that. To just check the quality of prep, I need a plasma membrane marker.
    • Our sodium potassium ATPase and plasma membrane products can be found using this links. I hope this helps. Please contact us at technical@novusbio.com with any additional questions.

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Bioinformatics

Gene Symbol ATP1A1
Entrez