Sodium Potassium ATPase Alpha 1 Antibody (EP1845Y)

Product Discontinued

Sodium Potassium ATPase Alpha 1 Antibody (EP1845Y) Summary

• Immunogen: A synthetic peptide corresponding to residues near the N-terminus of human Na+/K+ ATPase Alpha was used as an immunogen.
• Marker: Plasma Membrane Marker
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ATP1A1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:20
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-250
  • Western Blot 1:10000-50000
• Application Notes: This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Flow Cytometry. In Western blot this antibody detects a band at approximately 100kDa.
• Theoretical MW: 100 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Sodium Potassium ATPase Alpha 1 Overexpression Lysate

Reactivity Notes

Reacts with: Tilapia

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for Sodium Potassium ATPase Alpha 1 Antibody (EP1845Y)

• ATP1A1
• ATPase, Na+/K+ transporting, alpha 1 polypeptide
• EC 3.6.3
• EC 3.6.3.9
• EC 7.2.2.13
• K-ATPase alpha-1 subunit
• K-ATPase catalytic subunit alpha-A protein
• MGC3285
• MGC51750
• Na
• Na(+)/K(+) ATPase alpha-1 subunit
• Na, K-ATPase, alpha-A catalytic polypeptide
• Na+, K+ ATPase alpha subunit
• Na+/K+ ATPase 1
• Sodium Potassium ATPase Alpha 1
• sodium pump 1
• Sodium pump subunit alpha-1
• sodium/potassium-transporting ATPase subunit alpha-1
• sodium-potassium-ATPase, alpha 1 polypeptide

Background

Na+, K+-ATPase is a heterodimeric enzyme responsible for the active maintenance of sodium and potassium gradients across the plasma membrane (1). The sodium- and potassium-dependent adenosine triphosphatase (Na+,K(+)-ATPase) maintains the transmembrane Na+ gradient to which is coupled all active cellular transport systems (2). It is well accepted that inhibition of the Na,K-ATPase in the heart, through effects on the Na/Ca exchanger, raises the intracellular Ca2+ concentration and strengthens cardiac contraction. Assessing the phenotypes of mouse hearts with genetically reduced levels of Na,K-ATPase alpha 1 or alpha 2 isoforms clearly demonstrates different functional roles for these isoforms in vivo. Results definitively illustrate a specific role for the alpha 2 Na,K-ATPase isoform in Ca2+ signaling during cardiac contraction (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sodium Potassium ATPase Alpha 1 Antibody (NB100-80005)(6)

Publications using NB100-80005

• Cottrell GS, Soubrane CH, Hounshell JA et al. CACHD1 is an a2d-like protein that modulates CaV3 voltage-gated calcium channel activity. J. Neurosci. 2018-09-04 [PMID: 30181139] (WB, Rat)
• Hosoya M, Fujioka M, Ogawa K, Okano H et al. Distinct Expression Patterns Of Causative Genes Responsible For Hereditary Progressive Hearing Loss In Non-Human Primate Cochlea Sci Rep 2016-02-26 [PMID: 26915689] (IHC-Fr, Primate)
• Kim H-M, Wangemann P. Epithelial cell stretching and luminal acidification lead to a retarded development of stria vascularis and deafness in mice lacking pendrin. PLoS ONE. 2011-01-01 [PMID: 21423764]
• McIntosh AL, Storey SM, Atshaves BP. Intracellular lipid droplets contain dynamic pools of sphingomyelin: ADRP binds phospholipids with high affinity. Lipids 2010-06-01 [PMID: 20473576] (WB, Mouse)
• Daley WP, Gulfo KM, Sequeira SJ et al. Identification of a mechanochemical checkpoint and negative feedback loop regulating branching morphogenesis. Dev Biol 2009-12-01 [PMID: 19804774]
• Kada S, Nakagawa T, Ito J et al. A mouse model for degeneration of the spiral ligament. J Assoc Res Otolaryngol 2009-06-01 [PMID: 19212789] (IF/IHC)

FAQs for Sodium Potassium ATPase Alpha 1 Antibody (NB100-80005). (Showing 1 - 1 of 1 FAQs).

1. I am looking forward to pick up a plasma membrane marker. I have gastric cancer cell line and I am preparing PM out of that. To just check the quality of prep, I need a plasma membrane marker.
   • Our sodium potassium ATPase and plasma membrane products may be of use to you. Please contact us at technical@novusbio.com with any additional questions.

Bioinformatics

• Gene Symbol: ATP1A1
• Entrez:
  • 476(Human)
  • 11928(Mouse)
  • 24211(Rat)
• Uniprot:
  • P05023(Human)