SODD/BAG4 Antibody

Knockdown Validated: SODD/BAG4 Antibody [NB100-56464] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and SODD/BAG4 knockout (KO) HeLa cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human SODD/BAG4 Polyclonal Antibody (Catalog # NB100-56565) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for SODD/BAG4 at approximately 75 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.

Western Blot: SODD/BAG4 Antibody [NB100-56464] - Analysis of SODD/BAG4 in HeLa lysate using this antibody at 0.5 ug/ml.

Product Discontinued

SODD/BAG4 Antibody Summary

• Immunogen: Amino acids 2-16 (SALRRSGYGPSDGPSC) of human SODD protein were used as the immunogen.
• Specificity: The 60-65 kD band corresponding to SODD protein is blocked by immunizing peptide.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BAG4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Knockdown Validated
  • Western Blot 0.5-2 ug/ml
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.2% gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for SODD/BAG4 Antibody

• BAG4
• BAG-4
• BAG-family molecular chaperone regulator-4
• Bcl-2-associated athanogene 4
• BCL2-associated athanogene 4
• Silencer of death domains
• SODD
• SODDBAG family molecular chaperone regulator 4

Background

Several cell surface receptors that are involved in apoptosis contain intracellular death domains and are capable of triggering apoptosis when activated by their respective ligands (1). Due to presence of these death domains and presence of various stimuli in the serum as well as overexpression leading to receptor oligomerization, which leads to constitutive activation of the apoptotic pathway, it has been extremely difficult to generate permanent cell lines overexpressing specific receptors in culture. However, in normal states, this death domain activity is silenced presumably due to presence of other cellular factors. SODD (Silencer of Death Domain), a DR3 associated protein, was isolated by yeast two-hybrid interaction assays (2). SODD associates with the intracellular domains of TNF-R1, but not with those of TNF-R2, Fas, DR4, or DR5. Overexpression has been found to suppress the TNF induced cell death as well as NF-kB activation (2). SODD may act as a negative regulatory protein that is normally associated with death domain of TNF-R1 and probably inhibit self-oligomerization properties of death domain and maintain TNF-R1 in an inactive, monomeric state.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BAG4
• Entrez:
  • 67384(Mouse)